Development of an antigen Enzyme-Linked AptaSorbent Assay (ELASA) for the detection of swine influenza virus in field samples
Ver/Abrir
Autor/a
Aira, Cristina
Klett-Mingo, Jose Ignacio
Ruiz, Tamara
Garcia-Sacristán, Ana
Martín-Valls, Gerard Eduard
Mateu, Enric
Gómez-Laguna, Jaime
Rueda, Paloma
González, Víctor Manuel
Rodríguez, María José
López, Lissette
Fecha de publicación
2021-08-09ISSN
0003-2670
Resumen
Influenza viruses are highly variable pathogens that infect a wide range of mammalian and avian species. According to the internal conserved proteins (nucleoprotein: NP, and matrix proteins: M), these viruses are classified into type A, B, C, and D. Influenza A virus in swine is of significant importance to the industry since it is responsible for endemic infections that lead to high economic loses derived from poor weight gain, reproductive disorders, and the role it plays in Porcine Respiratory Disease Complex (PRDC). To date, swine influenza virus (SIV) diagnosis continues to be based in complex and expensive technologies such as RT-qPCR. In this study, we aimed to improve actual tools by the implementation of aptamers as capture molecules. First, three different aptamers have been selected using as target the recombinant NP of Influenza A virus expressed in insect cells. Then, these molecules have been used for the development of an Enzyme-Linked AptaSorbent Assay (ELASA) in combination with specific monoclonal antibodies for Influenza A detection. A total of 171 field samples (nasal swabs) have been evaluated with the newly developed assay obtaining a 79.7% and 98.1% sensitivity and specificity respectively, using real time RT-PCR as standard assay. These results suggest that the assay is a promising method that could be used for Influenza A detection in analysis laboratories facilitating surveillance labours.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
619 - Veterinaria
Páginas
8
Publicado por
Elsevier
Publicado en
Analytica Chimica Acta
Citación
Aira, Cristina, Jose Ignacio Klett-Mingo, Tamara Ruiz, Ana Garcia-Sacristán, Gerard Eduard Martín-Valls, Enric Mateu, and Jaime Gómez-Laguna et al. 2021. "Development Of An Antigen Enzyme-Linked Aptasorbent Assay (ELASA) For The Detection Of Swine Influenza Virus In Field Samples". Analytica Chimica Acta 1181: 338933. doi:10.1016/j.aca.2021.338933.
Número del acuerdo de la subvención
CDTI/EUROSTARS-2/E9781/ES/Development of versatile multiplex antigen diagnostic tests for relevant infections of swine/MultiDETECT
Program
Sanitat Animal
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2805]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by-nc-nd/4.0/