Optimization of a food industry-waste-based medium for the production of the plant growth promoting microorganism Pseudomonas oryzihabitans PGP01 based on agro-food industries by-products
Author
Solsona, Cristina
Vilanova, Laura
Publication date
2021-09-16ISSN
2215-017X
Abstract
In this study, three wastes based on potato peels and pulps, tomato seeds and wheat bran were used as basis for the preparation of a cheap medium to produce the bacterium P. oryzihabitans PGP01. In flasks experiments, P. oryzihabitans PGP01 growth at 25 °C in a medium based on frozen potato peels and pulp (FPP) with tryptone as a nitrogen source resulted in the maximum production compared to the commercial TSB medium. In the scale-up to 2 L bioreactors, FPP supplemented with tryptone, molasses, NaCl and K2HPO4 allowed to reach similar biomass production than in the TSB medium. A maximum growth of 4.4 × 109 CFU mL−1 after setting the agitation and the air flux conditions at 400 rpm and 0.75 vvm. Finally, P. oryzihabitans PGP01 growing in this optimized medium conserved its biological activity showing the expected effect in root development previously reported for this microorganism.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
631 - Agriculture in general
Pages
12
Publisher
Elsevier
Is part of
Biotechnology Reports
Citation
Cantabella, Daniel, Ramon Dolcet-Sanjuan, Cristina Solsona, Laura Vilanova, Rosario Torres, and Neus Teixidó. 2021. "Optimization Of A Food Industry-Waste-Based Medium For The Production Of The Plant Growth Promoting Microorganism Pseudomonas Oryzihabitans PGP01 Based On Agro-Food Industries By-Products". Biotechnology Reports 32: e00675. doi:10.1016/j.btre.2021.e00675.
Grant agreement number
EC/H2020/720719/EU/Agri and food waste valorisation co-ops based on flexible multi-feedstocks biorefinery processing technologies for new high added value applications/AgriMax
Program
Fructicultura
Postcollita
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2831]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/