Malignant infarction of the middle cerebral artery in a porcine model. A pilot study
Visualitza/Obre
Autor/a
Arikan, Fuat
Martínez-Valverde, Tamara
Sánchez-Guerrero, Ángela
Campos, Mireia
Esteves, Marielle
Gandara, Dario
Torné, Ramon
Castro, Lidia
Tibau, Joan
Sahuquillo, Juan
Data de publicació
2017-02-24ISSN
1932-6203
Resum
Background and purpose. Interspecies variability and poor clinical translation from rodent studies indicate that large gyrencephalic animal stroke models are urgently needed. We present a proof-of-principle study describing an alternative animal model of malignant infarction of the middle cerebral artery (MCA) in the common pig and illustrate some of its potential applications. We report on metabolic patterns, ionic profile, brain partial pressure of oxygen (PtiO2), expression of sulfonylurea receptor 1 (SUR1), and the transient receptor potential melastatin 4 (TRPM4). Methods. A 5-hour ischemic infarct of the MCA territory was performed in 5 2.5-to-3-month-old female hybrid pigs (Large White x Landrace) using a frontotemporal approach. The core and penumbra areas were intraoperatively monitored to determine the metabolic and ionic profiles. To determine the infarct volume, 2,3,5-triphenyltetrazolium chloride staining and immunohistochemistry analysis was performed to determine SUR1 and TRPM4 expression.
Results. PtiO2 monitoring showed an abrupt reduction in values close to 0 mmHg after MCA occlusion in the core area. Hourly cerebral microdialysis showed that the infarcted tissue was characterized by reduced concentrations of glucose (0.03 mM) and pyruvate (0.003 mM) and increases in lactate levels (8.87mM), lactate-pyruvate ratio (4202), glycerol levels (588 μM), and potassium concentration (27.9 mmol/L). Immunohistochemical analysis showed increased expression of SUR1-TRPM4 channels.
Conclusions. The aim of the present proof-of-principle study was to document the feasibility of a large animal model of malignant MCA infarction by performing transcranial occlusion of the MCA in the common pig, as an alternative to lisencephalic animals. This model may be useful for detailed studies of cerebral ischemia mechanisms and the development of neuroprotective strategies.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
21
Publicat per
Public Library of Science
Publicat a
PLoS ONE
Citació
Arikan, Fuat, Tamara Martínez-Valverde, Ángela Sánchez-Guerrero, Mireia Campos, Marielle Esteves, Dario Gandara, and Ramon Torné et al. 2017. "Malignant Infarction Of The Middle Cerebral Artery In A Porcine Model. A Pilot Study". PLOS ONE 12 (2): e0172637. doi:10.1371/journal.pone.0172637.
Número de l'acord de la subvenció
ISCIII-FEDER/ /FIS PI11-00700/ES/ /
Programa
Genètica i Millora Animal
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