Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
Visualitza/Obre
Autor/a
Olesen, Ann Sofie
Kodama, Miyako
Lohse, Louise
Rasmussen, Thomas Bruun
Lazov, Christina M.
Limborg, Morten T.
Gilbert, M. Thomas P.
Bøtner, Anette
Belsham, Graham J.
Data de publicació
2021-11-22ISSN
1999-4915
Resum
African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
15
Publicat per
MDPI
Publicat a
Viruses
Citació
Olesen, Ann Sofie, Miyako Kodama, Louise Lohse, Francesc Accensi, Thomas Bruun Rasmussen, Christina M. Lazov, Morten T. Limborg, M. Thomas P. Gilbert, Anette Bøtner, and Graham J. Belsham. 2021. "Identification Of African Swine Fever Virus Transcription Within Peripheral Blood Mononuclear Cells Of Acutely Infected Pigs". Viruses 13 (11): 2333. doi:10.3390/v13112333.
Programa
Sanitat Animal
Aquest element apareix en la col·lecció o col·leccions següent(s)
- ARTICLES CIENTÍFICS [2549]
Els següents fitxers sobre la llicència estan associats a aquest element:
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com http://creativecommons.org/licenses/by/4.0/