Identification of African Swine Fever Virus Transcription within Peripheral Blood Mononuclear Cells of Acutely Infected Pigs
Ver/Abrir
Autor/a
Olesen, Ann Sofie
Kodama, Miyako
Lohse, Louise
Rasmussen, Thomas Bruun
Lazov, Christina M.
Limborg, Morten T.
Gilbert, M. Thomas P.
Bøtner, Anette
Belsham, Graham J.
Fecha de publicación
2021-11-22ISSN
1999-4915
Resumen
African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs has not been well characterized previously. In this study, four pigs were infected with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples were collected before inoculation and at both 3 and 6 days later. During this period, a range of clinical signs of infection became apparent in the pigs. From the blood, peripheral blood mononuclear cells (PBMCs) were isolated. The transcription of the ASFV genes was determined using RNAseq on poly(A)+ mRNAs isolated from these cells. Only very low levels of virus transcription were detected in the PBMCs at 3 days post-inoculation (dpi) but, at 6 dpi, extensive transcription was apparent. This was co-incident with a large increase in the level of ASFV DNA within these cells. The pattern of the virus gene expression was very reproducible between the individual pigs. Many highly expressed genes have undefined roles. Surprisingly, some genes with key roles in virus replication were expressed at only low levels. As the functions of individual genes are identified, information about their expression becomes important for understanding their contribution to virus biology.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
15
Publicado por
MDPI
Publicado en
Viruses
Citación
Olesen, Ann Sofie, Miyako Kodama, Louise Lohse, Francesc Accensi, Thomas Bruun Rasmussen, Christina M. Lazov, Morten T. Limborg, M. Thomas P. Gilbert, Anette Bøtner, and Graham J. Belsham. 2021. "Identification Of African Swine Fever Virus Transcription Within Peripheral Blood Mononuclear Cells Of Acutely Infected Pigs". Viruses 13 (11): 2333. doi:10.3390/v13112333.
Program
Sanitat Animal
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2541]
El ítem tiene asociados los siguientes ficheros de licencia:
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/