Genome-Wide Association Study Singles Out SCD and LEPR as the Two Main Loci Influencing Intramuscular Fat Content and Fatty Acid Composition in Duroc Pigs
Ver/Abrir
Autor/a
Ros-Freixedes, Roger
Gol, Sofia
Pena, Ramona N.
Tor, Marc
Ibáñez-Escriche, Noelia
Dekkers, Jack C. M.
Estany, Joan
Fecha de publicación
2016-03-29ISSN
1932-6203
Resumen
Intramuscular fat (IMF) content and fatty acid composition affect the organoleptic quality and nutritional value of pork. A genome-wide association study was performed on 138 Duroc pigs genotyped with a 60k SNP chip to detect biologically relevant genomic variants influencing fat content and composition. Despite the limited sample size, the genome-wide association study was powerful enough to detect the association between fatty acid composition and a known haplotypic variant in SCD (SSC14) and to reveal an association of IMF and fatty acid composition in the LEPR region (SSC6). The association of LEPR was later validated with an independent set of 853 pigs using a candidate quantitative trait nucleotide.
The SCD gene is responsible for the biosynthesis of oleic acid (C18:1) from stearic acid. This locus affected the stearic to oleic desaturation index (C18:1/C18:0), C18:1, and saturated (SFA) and monounsaturated (MUFA) fatty acids content. These effects were consistently detected in gluteus medius, longissimus dorsi, and subcutaneous fat. The association of LEPR with fatty acid composition was detected only in muscle and was, at least in part, a consequence of its effect on IMF content, with increased IMF resulting in more SFA, less polyunsaturated fatty acids (PUFA), and greater SFA/PUFA ratio. Marker substitution effects estimated with a subset of 65 animals were used to predict the genomic
estimated breeding values of 70 animals born 7 years later. Although predictions with the whole SNP chip information were in relatively high correlation with observed SFA, MUFA, and C18:1/C18:0 (0.48–0.60), IMF content and composition were in general better predicted by using only SNPs at the SCD and LEPR loci, in which case the correlation between predicted and observed values was in the range of 0.36 to 0.54 for all traits. Results indicate that markers in the SCD and LEPR genes can be useful to select for optimum fatty acid profiles of pork.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
636 - Explotación y cría de animales. Cría del ganado y de animales domésticos
Páginas
18
Publicado por
Public Library of Science
Publicado en
PLoS ONE
Citación
Ros-Freixedes, Roger, Sofia Gol, Ramona N. Pena, Marc Tor, Noelia Ibáñez-Escriche, Jack C. M. Dekkers, and Joan Estany. 2016. "Genome-Wide Association Study Singles Out SCD And LEPR As The Two Main Loci Influencing Intramuscular Fat Content And Fatty Acid Composition In Duroc Pigs". PLOS ONE 11 (3): e0152496. doi:10.1371/journal.pone.0152496.
Número del acuerdo de la subvención
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-33529/ES/MEJORA DE LA CALIDAD DE LA CARNE DE CERDO MEDIANTE EL INCREMENTO DE SU CONTENIDO EN ACIDO OLEICO/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-65846-R/ES/REGULACION NUTRIGENETICA DEL CONTENIDO Y LA COMPOSICION DE LA GRASA EN EL CERDO/
Program
Genètica i Millora Animal
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2802]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/