Antimicrobial Resistant Salmonella in Chelonians: Assessing Its Potential Risk in Zoological Institutions in Spain
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Author
Marin, Clara
Martín-Maldonado, Bárbara
Sevilla-Navarro, Sandra
Lorenzo-Rebenaque, Laura
Montoro-Dasi, Laura
Manzanares, Alicia
Ayats, Teresa
Mencía-Gutiérrez, Aida
Jordá, Jaume
González, Fernando
Rojo-Solís, Carlos
Barros, Carlos
García-Párraga, Daniel
Vega, Santiago
Publication date
2022-05-31ISSN
2306-7381
Abstract
Salmonella is mostly noted as a food-borne pathogen, but contact with chelonians has also
been reported as a source of infection. Moreover, high levels of antimicrobial resistance (AMR) have
been reported in Salmonella isolated from wild and captive reptiles. The aim of this study was to assess
the occurrence of Salmonella AMR carriage by chelonians admitted to two zoological institutions
in Spain, characterizing the isolates to assess the Salmonella AMR epidemiology in wildlife. To this
end, 152 chelonians from nine species were sampled upon their arrival at the zoological nuclei.
Salmonella identification was based on ISO 6579-1:2017 (Annex D), isolates were serotyped and their
AMR analysed according to the EU Decision 2013/652. Moreover, the genetic relationship of the
isolates was assessed by pulsed-field gel electrophoresis (PFGE). Results showed 19% (29/152) of the
chelonians positive to Salmonella, all of them tortoises. For all isolates, 69% (20/29) were resistant
and 34% (10/29) multidrug-resistant (MDR) strains. PFGE clustered isolates according to the serovar,
confirming a low genetic diversity. In conclusion, this study shows a high presence of MDR Salmonella
strains in tortoises at their entry into zoological nuclei. This condition highlights the need to establish
Salmonella detection protocols for the entry of animals into these centres.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
12
Publisher
MDPI
Is part of
Veterinary Sciences
Citation
Marin, Clara, Bárbara Martín-Maldonado, Marta Cerdà-Cuéllar, Sandra Sevilla-Navarro, Laura Lorenzo-Rebenaque, Laura Montoro-Dasi, Alicia Manzanares, Teresa Ayats, Aida Mencía-Gutiérrez, Jaume Jordá, Fernando González, Carlos Rojo-Solís, Carlos Barros, Daniel García-Párraga and Santiago Vega. 2022. "Antimicrobial Resistant Salmonella In Chelonians: Assessing Its Potential Risk In Zoological Institutions In Spain". Veterinary Sciences 9 (6): 264. doi:10.3390/vetsci9060264.
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2685]
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