QTL mapping of melon fruit quality traits using a high-density GBS-based genetic map
Ver/Abrir
Autor/a
Pereira, L.
Ruggieri, V.
Pérez, S.
Alexiou, K. G.
Fernández, M.
Jahrmann, T.
Pujol, M.
Garcia-Mas, J.
Fecha de publicación
2018-12-04ISSN
1471-2229
Resumen
Background
Melon shows a broad diversity in fruit morphology and quality, which is still underexploited in breeding programs. The knowledge of the genetic basis of fruit quality traits is important for identifying new alleles that may be introduced in elite material by highly efficient molecular breeding tools.
Results
In order to identify QTLs controlling fruit quality, a recombinant inbred line population was developed using two commercial cultivars as parental lines: “Védrantais”, from the cantalupensis group, and “Piel de Sapo”, from the inodorus group. Both have desirable quality traits for the market, but their fruits differ in traits such as rind and flesh color, sugar content, ripening behavior, size and shape. We used a genotyping-by-sequencing strategy to construct a dense genetic map, which included around five thousand variants distributed in 824 bins. The RIL population was phenotyped for quality and morphology traits, and we mapped 33 stable QTLs involved in sugar and carotenoid content, fruit and seed morphology and major loci controlling external color of immature fruit and mottled rind. The median confidence interval of the QTLs was 942 kb, suggesting that the high density of the genetic map helped in increasing the mapping resolution. Some of these intervals contained less than a hundred annotated genes, and an integrative strategy combining gene expression and resequencing data enabled identification of candidate genes for some of these traits.
Conclusion
Several QTLs controlling fruit quality traits in melon were identified and delimited to narrow genomic intervals, using a RIL population and a GBS-based genetic map.
Keywords
Quantitative trait loci
Melon
Fruit quality
Fruit morphology
Genotyping-by-sequencing
Genetic map
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
633 - Cultius i produccions
Páginas
17
Publicado por
BMC
Publicado en
BMC Plant Biology
Citación
Pereira, L., V. Ruggieri, S. Pérez, K. G. Alexiou, M. Fernández, T. Jahrmann, M. Pujol, and J. Garcia-Mas. 2018. "QTL Mapping Of Melon Fruit Quality Traits Using A High-Density GBS-Based Genetic Map". BMC Plant Biology 18 (1). Springer Nature. doi:10.1186/s12870-018-1537-5.
Número del acuerdo de la subvención
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-64625-C2-1-R/ES/DISECCION GENETICA DE DOS CARACTERES DE INTERES AGRONOMICO EN MELON: RESISTENCIA A CUCUMBER MOSAIC VIRUS Y MADURACION CLIMATERICA DE FRUTO/RESMELORIP
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
EC/H2020/665919/EU/Opening Sphere UAB-CEI to PostDoctoral Fellows/P-SPHERE
Program
Genòmica i Biotecnologia
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