Agreement and differential use of laboratory methods for the detection and quantification of SARS-CoV-2 in experimentally infected animals
Visualitza/Obre
Autor/a
Lorca-Oró, Cristina
Roca, Núria
Pérez, Mónica
Carrillo, Jorge
Izquierdo-Useros, Nuria
Blanco, Julià
Clotet, Bonaventura
Vergara-Alert, Júlia
Data de publicació
2022-11-15ISSN
1664-302X
Resum
Rodents are widely used for the development of COVID-19-like animal models, the virological outcome being determined through several laboratory methods reported in the literature. Our objective was to assess the agreement between methods performed on different sample types from 342 rodents experimentally infected with SARS-CoV-2 (289 golden Syrian hamsters and 53 K18-hACE2 mice). Our results showed moderate agreement between methods detecting active viral replication, and that increasing viral loads determined by either RT-qPCR or infectious viral titration corresponded to increasing immunohistochemical scores. The percentage of agreement between methods decreased over experimental time points, and we observed poor agreement between RT-qPCR results and viral titration from oropharyngeal swabs. In conclusion, RT-qPCR and viral titration on tissue homogenates are the most reliable techniques to determine the presence and replication of SARS-CoV-2 in the early and peak phases of infection, and immunohistochemistry is valuable to evaluate viral distribution patterns in the infected tissues.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
10
Publicat per
Frontiers Media
Publicat a
Frontiers in Microbiology
Citació
Usai, Carla, Lola Pailler-García, Cristina Lorca-Oró, Leira Fernández-Bastit, Núria Roca, Marco Brustolin, and Jordi Rodon et al. 2022. "Agreement And Differential Use Of Laboratory Methods For The Detection And Quantification Of SARS-Cov-2 In Experimentally Infected Animals". Frontiers In Microbiology 13. doi:10.3389/fmicb.2022.1016201.
Número de l'acord de la subvenció
MICINN/Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-117145RB-I00/ES/NUEVAS TERAPIAS ANTIVIRALES E INMUNOMODULADORAS FRENTE AL SARS-COV-2/
EU/H2020/101046118/EC/RBD Dimer recombinant protein vaccine against SARSCoV2/RBDCOV
Programa
Sanitat Animal
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