Cell immunolocalization of ciguatoxin-like compounds in the benthic dinoflagellate Gambierdiscus australes M. Chinain & M.A. Faust by confocal microscopy
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Autor/a
Honsell, Giorgio
Hirama, Masahiro
Pelin, Marco
Tubaro, Aurelia
Tsumuraya, Takeshi
Data de publicació
2022-11-23ISSN
1568-9883
Resum
Dinoflagellates of the genera Gambierdiscus and Fukuyoa are able to produce potent neurotoxins like ciguatoxins (CTXs), which, after biooxidation in fish, are responsible for ciguatera intoxication. An isolate of G. australes from the Canary Islands, that revealed the presence of CTX-like compounds by immunosensing tools, was studied by immunocytochemistry to localize intracellular CTX-like compounds, using 8H4 monoclonal antibody that specifically recognizes the right wing of CTX1B and CTX3C analogues. Confocal microscopy observations of immunostained whole cells revealed a strong positive reaction on cell surface and all along the cell outline, while no reaction was detected inside the cells, probably because the antibody was not able to pass through thecal plates. Cell sections showed a positive antibody staining not only on thecal plates, but also inside cytoplasm, with numerous small dots and larger tubule-like reticulate structures. Small fluorescent dots were detected also on the nuclear surface. These observations indicate that CTX-like compounds are present in G. australes cytoplasm, and then are, at least in part, released to cover the cell surface.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
574 - Ecologia general i biodiversitat
Pàgines
25
Publicat per
Elsevier
Publicat a
Harmful Algae
Citació
Honsell, Giorgio, Greta Gaiani, Masahiro Hirama, Marco Pelin, Aurelia Tubaro, Takeshi Tsumuraya, and Mònica Campàs. 2022. "Cell Immunolocalization Of Ciguatoxin-Like Compounds In The Benthic Dinoflagellate Gambierdiscus Australes M. Chinain &Amp; M.A. Faust By Confocal Microscopy". Harmful Algae 120: 102353. doi:10.1016/j.hal.2022.102353.
Número de l'acord de la subvenció
EC/H2020/778069/EU/Emergent Marine Toxins in the North Atlantic and Mediterranean: New Approaches to Assess their Occurrence and Future Scenarios in the Framework of Global Environmental Changes/EMERTOX
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
Programa
Aigües Marines i Continentals
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