An LTR retrotransposon in the promoter of a PsMYB10.2 gene associated with the regulation of fruit f lesh color in Japanese plum
Visualitza/Obre
Autor/a
Fiol, Arnau
García, Sergio
Dujak, Christian
Pacheco, Igor
Infante, Rodrigo
Data de publicació
2022-09-13ISSN
2052-7276
Resum
Japanese plums exhibit wide diversity of fruit coloration. The red to black hues are caused by the accumulation of anthocyanins, while their absence results in yellow, orange or green fruits. In Prunus, MYB10 genes are determinants for anthocyanin accumulation. In peach, QTLs for red plant organ traits map in an LG3 region with three MYB10 copies (PpMYB10.1, PpMYB10.2 and PpMYB10.3). In Japanese plum the gene copy number in this region differs with respect to peach: there are at least three copies of PsMYB10.1, with the expression of one of them (PsMYB10.1a) correlating with fruit skin color. The objective of this study was to determine a possible role of LG3-PsMYB10 genes in the natural variability of the f lesh color trait and to develop a molecular marker for marker-assisted selection (MAS). We
explored the variability within the LG3-PsMYB10 region using long-range sequences obtained in previous studies through CRISPR-Cas9 enrichment sequencing. We found that the PsMYB10.2 gene was only expressed in red f lesh fruits. Its role in promoting anthocyanin biosynthesis was validated by transient overexpression in Japanese plum fruits. The analysis of long-range sequences identified an LTR retrotransposon in the promoter of the expressed PsMYB10.2 gene that explained the trait in 93.1% of the 145 individuals analyzed. We hypothesize that the LTR retrotransposon may promote the PsMYB10.2 expression and activate the anthocyanin biosynthesis pathway. We propose for the first time the PsMYB10.2 gene as candidate for the f lesh color natural variation in Japanese plum and provide a
molecular marker for MAS.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
633 - Cultius i produccions
Pàgines
12
Publicat per
Oxford University Press
Publicat a
Horticulture Research
Citació
Fiol, Arnau, Sergio García, Christian Dujak, Igor Pacheco, Rodrigo Infante, and Maria José Aranzana. 2022. "An LTR Retrotransposon In The Promoter Of A Psmyb10.2 Gene Associated With The Regulation Of Fruit Flesh Color In Japanese Plum". Horticulture Research 9. doi:10.1093/hr/uhac206.
Número de l'acord de la subvenció
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-100795-B-I00/ES/HERRAMIENTAS MOLECULARES PARA LA MEJORA GENETICA DE CARACTERES DE CALIDAD EN MELOCOTONERO Y OTROS PRUNUS/
MC/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-128885OB-I00/ES/ /
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /
FEDER/ / /EU/ /
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
Programa
Genòmica i Biotecnologia
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