P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection
Visualitza/Obre
Autor/a
Barral, Thiago Doria
Infantes-Lorenzo, José A.
Moreno, Inmaculada
de Garnica García, M. Gracia
Gortázar, Christian
Meyer, Roberto
Portela, Ricardo D.
Domínguez, Lucas
Domínguez, Mercedes
Balseiro, Ana
Data de publicació
2022-10-07ISSN
0147-9571
Resum
The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
26
Publicat per
Elsevier
Publicat a
Comparative Immunology, Microbiology and Infectious Diseases
Citació
Barral, Thiago Doria, José A. Infantes-Lorenzo, Inmaculada Moreno, M. Gracia de Garnica García, Bernat Pérez de Val, Christian Gortázar, and Roberto Meyer et al. 2022. "P22 Protein Complex In The Serodiagnosis Of Animal Tuberculosis: Antigenic Stability And Cross-Reactivity With Corynebacterium Pseudotuberculosis Infection". Comparative Immunology, Microbiology And Infectious Diseases 90-91: 101891. doi:10.1016/j.cimid.2022.101891.
Número de l'acord de la subvenció
MICIU-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-096010-B-C21/ES/El tejón (Meles meles) y la tuberculosis animal en España: interacción tejón-bovino en hospot areas y medidas de control de la enfermedad en la interfase/
Programa
Sanitat Animal
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