P22 protein complex in the serodiagnosis of animal tuberculosis: Antigenic stability and cross-reactivity with Corynebacterium pseudotuberculosis infection
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Author
Barral, Thiago Doria
Infantes-Lorenzo, José A.
Moreno, Inmaculada
de Garnica García, M. Gracia
Gortázar, Christian
Meyer, Roberto
Portela, Ricardo D.
Domínguez, Lucas
Domínguez, Mercedes
Balseiro, Ana
Publication date
2022-10-07ISSN
0147-9571
Abstract
The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
26
Publisher
Elsevier
Is part of
Comparative Immunology, Microbiology and Infectious Diseases
Citation
Barral, Thiago Doria, José A. Infantes-Lorenzo, Inmaculada Moreno, M. Gracia de Garnica García, Bernat Pérez de Val, Christian Gortázar, and Roberto Meyer et al. 2022. "P22 Protein Complex In The Serodiagnosis Of Animal Tuberculosis: Antigenic Stability And Cross-Reactivity With Corynebacterium Pseudotuberculosis Infection". Comparative Immunology, Microbiology And Infectious Diseases 90-91: 101891. doi:10.1016/j.cimid.2022.101891.
Grant agreement number
MICIU-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-096010-B-C21/ES/El tejón (Meles meles) y la tuberculosis animal en España: interacción tejón-bovino en hospot areas y medidas de control de la enfermedad en la interfase/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2802]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/