Physicochemical and Biochemical Properties of Trypsin-like Enzyme from Two Sturgeon Species
Autor/a
Zamani, Abbas
Khajavi, Maryam
Kenari, Abdolmohammad Abedian
Nazarpak, Masoumeh Haghbin
Solouk, Atefeh
Esmaeili, Mini
Fecha de publicación
2023-02-26ISSN
2076-2615
Resumen
This work aimed to determine the physicochemical and biochemical properties of trypsin from beluga Huso huso and sevruga Acipenser stellatus, two highly valuable sturgeon species. According to the results obtained from the methods of casein-zymogram and inhibitory activity staining, the molecular weight of trypsin for sevruga and beluga was 27.5 and 29.5 kDa, respectively. Optimum pH and temperature values for both trypsins were recorded at 8.5 and 55 °C by BAPNA (a specific substrate), respectively. The stability of both trypsins was well-preserved at pH values from 6.0 to 11.0 and temperatures up to 50 °C. TLCK and SBTI, two specific trypsin inhibitors, showed a significant inhibitory effect on the enzymatic activity of both trypsins (p < 0.05). The enzyme activity was significantly increased in the presence of Ca+2 and surfactants and decreased by oxidizing agents, Cu+2, Zn+2, and Co+2 (p < 0.05). However, univalent ions Na+ and K+ did not show any significant effect on the activity of both trypsins (p > 0.05). The results of our study show that the properties of trypsin from beluga and sevruga are in agreement with data reported in bony fish and can contribute to the clear understanding of trypsin activity in these primitive species.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
637 - Productos de los animales domésticos, de la caza y de la pesca
Páginas
15
Publicado por
MDPI
Publicado en
Animals
Citación
Zamani, Abbas, Maryam Khajavi, Abdolmohammad Abedian Kenari, Masoumeh Haghbin Nazarpak, Atefeh Solouk, Mina Esmaeili, and Enric Gisbert. 2023. "Physicochemical And Biochemical Properties Of Trypsin-Like Enzyme From Two Sturgeon Species". Animals 13 (5): 853. doi:10.3390/ani13050853.
Program
Aqüicultura
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