Plant Growth Stimulation and Root Colonization Potential of In Vivo versus In Vitro Arbuscular Mycorrhizal Inocula
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Publication date
2013-07ISSN
0018-5345
Abstract
Inoculum of arbuscular mycorrhizal fungi, with growing use in horticulture, is
produced mainly in two technically different cultivation systems: in vivo culture in
symbiosis with living host plants or in vitro culture in which the fungus life cycle develops
in association with transformed roots. To evaluate the effectiveness and the infectivity
of a defined isolate obtained by both production methods, a replicated comparative
evaluation experiment was designed using different propagules of Rhizophagus irregularis produced in vivo on leek plants or in vitro in monoxenic culture on transformed
carrot roots. The size of the spores obtained under both cultivation methods was first
assessed and bulk inoculum, spores, sievings, and mycorrhizal root fragments were used
to inoculate leek plantlets. Spores produced in vitro were significantly smaller than those
produced in vivo. Although all mycorrhizal propagules used as a source of inoculum were
able to colonize plants, in all cases, leek plants inoculated with propagules obtained in
vivo achieved significantly higher mycorrhizal colonization rates than plants inoculated
with in vitro inocula. Inoculation with in vivo bulk inoculum and in vivo mycorrhizal root
fragments were the only treatments increasing plant growth. These results indicate that
the production system of arbuscular mycorrhizal fungi itself can have implications in the
stimulation of plant growth and in experimental results.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
632 - Plant damage, injuries. Plant diseases. Pests, organisms injurious to plants. Plant protection
Pages
5
Publisher
American Society for Horticultural Science
Is part of
HortScience
Citation
Calvet, Cinta, Amelia Camprubi, Ana Pérez-Hernández, and Paulo Emilio Lovato. 2013. "Plant Growth Stimulation And Root Colonization Potential Of In Vivo Versus In Vitro Arbuscular Mycorrhizal Inocula". Hortscience 48 (7): 897-901. doi:10.21273/hortsci.48.7.897.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-15017/ES/DESAROLLO BIOTECNOLOGICO DE LOS PROCESOS DE MICORRIZACION Y DE TRAZABILIDAD PARA LA GESTION SOSTENIBLE DE EXPLOTACIONES AGRARIAS Y DE ENTORNOS PAISAJISTICOS/
Program
Protecció Vegetal Sostenible
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2661]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/