Plant Growth Stimulation and Root Colonization Potential of In Vivo versus In Vitro Arbuscular Mycorrhizal Inocula
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Fecha de publicación
2013-07ISSN
0018-5345
Resumen
Inoculum of arbuscular mycorrhizal fungi, with growing use in horticulture, is
produced mainly in two technically different cultivation systems: in vivo culture in
symbiosis with living host plants or in vitro culture in which the fungus life cycle develops
in association with transformed roots. To evaluate the effectiveness and the infectivity
of a defined isolate obtained by both production methods, a replicated comparative
evaluation experiment was designed using different propagules of Rhizophagus irregularis produced in vivo on leek plants or in vitro in monoxenic culture on transformed
carrot roots. The size of the spores obtained under both cultivation methods was first
assessed and bulk inoculum, spores, sievings, and mycorrhizal root fragments were used
to inoculate leek plantlets. Spores produced in vitro were significantly smaller than those
produced in vivo. Although all mycorrhizal propagules used as a source of inoculum were
able to colonize plants, in all cases, leek plants inoculated with propagules obtained in
vivo achieved significantly higher mycorrhizal colonization rates than plants inoculated
with in vitro inocula. Inoculation with in vivo bulk inoculum and in vivo mycorrhizal root
fragments were the only treatments increasing plant growth. These results indicate that
the production system of arbuscular mycorrhizal fungi itself can have implications in the
stimulation of plant growth and in experimental results.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
632 - Enfermedades y protección de las plantas
Páginas
5
Publicado por
American Society for Horticultural Science
Publicado en
HortScience
Citación
Calvet, Cinta, Amelia Camprubi, Ana Pérez-Hernández, and Paulo Emilio Lovato. 2013. "Plant Growth Stimulation And Root Colonization Potential Of In Vivo Versus In Vitro Arbuscular Mycorrhizal Inocula". Hortscience 48 (7): 897-901. doi:10.21273/hortsci.48.7.897.
Número del acuerdo de la subvención
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-15017/ES/DESAROLLO BIOTECNOLOGICO DE LOS PROCESOS DE MICORRIZACION Y DE TRAZABILIDAD PARA LA GESTION SOSTENIBLE DE EXPLOTACIONES AGRARIAS Y DE ENTORNOS PAISAJISTICOS/
Program
Protecció Vegetal Sostenible
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