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Safety and Efficacy upon Infection in Sheep with Rift Valley Fever Virus ZH548-rA2, a Triple Mutant Rescued Virus
dc.contributor.author | Moreno, Sandra | |
dc.contributor.author | Lorenzo, Gema | |
dc.contributor.author | López Valiñas, Álvaro | |
dc.contributor.author | de la Losa, Nuria | |
dc.contributor.author | Alonso, Celia | |
dc.contributor.author | Charro, Elena | |
dc.contributor.author | Núñez, José I. | |
dc.contributor.author | Sánchez-Cordón, Pedro J. | |
dc.contributor.author | Borrego, Belén | |
dc.contributor.author | Brun, Alejandro | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2024-02-16T08:21:15Z | |
dc.date.available | 2024-02-16T08:21:15Z | |
dc.date.issued | 2024-01-05 | |
dc.identifier.citation | Moreno, Sandra, Gema Lorenzo, Álvaro López-Valiñas, Nuria De La Losa, Celia Alonso, Elena Charro, José I. Núñez, P.J. Sánchez-Cordón, Belén Borrego, and Alejandro Brun. 2024. “Safety and Efficacy upon Infection in Sheep with Rift Valley Fever Virus ZH548-rA2, a Triple Mutant Rescued Virus.” Viruses 16 (1): 87. doi:10.3390/v16010087. | ca |
dc.identifier.issn | 1999-4915 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2818 | |
dc.description.abstract | The introduction of three single nucleotide mutations into the genome of the virulent RVFV ZH548 strain allows for the rescue of a fully attenuated virus in mice (ZH548-rA2). These mutations are located in the viral genes encoding the RdRp and the non-structural protein NSs. This paper shows the results obtained after the subcutaneous inoculation of ZH548-rA2 in adult sheep and the subsequent challenge with the parental virus (ZH548-rC1). Inoculation with the ZH548-rA2 virus caused no detectable clinical or pathological effect in sheep, whereas inoculation of the parental rC1 virus caused lesions compatible with viral infection characterised by the presence of scattered hepatic necrosis. Viral infection was confirmed via immunohistochemistry, with hepatocytes within the necrotic foci appearing as the main cells immunolabelled against viral antigen. Furthermore, the inoculation of sheep with the rA2 virus prevented the liver damage expected after rC1 virus inoculation, suggesting a protective efficacy in sheep which correlated with the induction of both humoral and cell-mediated immune responses. | ca |
dc.description.sponsorship | This research was funded by grants AGL2017-83226-R and PID2021-122567OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF “A way of making Europe”. | ca |
dc.format.extent | 14 | ca |
dc.language.iso | eng | ca |
dc.publisher | MDPI | ca |
dc.relation.ispartof | Viruses | ca |
dc.rights | Attribution 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Safety and Efficacy upon Infection in Sheep with Rift Valley Fever Virus ZH548-rA2, a Triple Mutant Rescued Virus | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.relation.projectID | INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-83226-R/ES/UTILIZACION DEL VIRUS DE LA FIEBRE DEL VALLE DEL RIFT COMO VECTOR VACUNAL: MEJORA RACIONAL DE SU SEGURIDAD, ESTABILIDAD Y TRAZABILIDAD/ | ca |
dc.relation.projectID | FEDER/ / /EU/ / | ca |
dc.relation.projectID | MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-122567OB-I00/ES/DISEÑO DE UNA PLATAFORMA VACUNAL MULTIVALENTE BASADA EN LA VARIANTE 40FP8 DEL VIRUS DE LA FIEBRE DEL VALLE DEL RIFT/ | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.3390/v16010087 | ca |
dc.contributor.group | Sanitat Animal | ca |
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