SNP variation in the promoter of the PRKAG3 gene and association with meat quality traits in pig
Visualitza/Obre
Autor/a
Ryan, Marion T.
Hamill, Ruth M.
O’Halloran, Aisling M.
Davey, Grace C.
McBryan, Jean
Mullen, Anne M.
McGee, Chris
Southwood, Olwen I.
Sweeney, Torres
Data de publicació
2012-07-25ISSN
1471-2156
Resum
Background: The PRKAG3 gene encodes the γ3 subunit of adenosine monophosphate activated protein kinase
(AMPK), a protein that plays a key role in energy metabolism in skeletal muscle. Non-synonymous single nucleotide
polymorphisms (SNPs) in this gene such as I199V are associated with important pork quality traits. The objective of
this study was to investigate the relationship between gene expression of the PRKAG3 gene, SNP variation in the
PRKAG3 promoter and meat quality phenotypes in pork.
Results: PRKAG3 gene expression was found to correlate with a number of traits relating to glycolytic potential (GP)
and intramuscular fat (IMF) in three phenotypically diverse F1 crosses comprising of 31 Large White, 23 Duroc and
32 Pietrain sire breeds. The majority of associations were observed in the Large White cross. There was a significant
association between genotype at the g.-311A>G locus and PRKAG3 gene expression in the Large White cross. In
the same population, ten novel SNPs were identified within a 1.3 kb region spanning the promoter and from this
three major haplotypes were inferred. Two tagging SNPs (g.-995A>G and g.-311A>G) characterised the haplotypes
within the promoter region being studied. These two SNPs were subsequently genotyped in larger populations
consisting of Large White (n = 98), Duroc (n = 99) and Pietrain (n = 98) purebreds. Four major haplotypes including
promoter SNP’s g.-995A>G and g.-311A>G and I199V were inferred. In the Large White breed, HAP1 was associated
with IMF% in the M. longissmus thoracis et lumborum (LTL) and driploss%. HAP2 was associated with IMFL%
GP-influenced traits pH at 24 hr in LTL (pHULT), pH at 45 min in LTL (pH45LT) and pH at 45 min in the M.
semimembranosus muscle (pH45SM). HAP3 was associated with driploss%, pHULT pH45LT and b* Minolta. In the
Duroc breed, associations were observed between HAP1 and driploss% and pHUSM. No associations were observed
with the remaining haplotypes (HAP2, HAP3 and HAP4) in the Duroc breed. The Pietrain breed was monomorphic
in the promoter region. The I199V locus was associated with several GP-influenced traits across all three breeds and
IMF% in the Large White and Pietrain breed. No significant difference in promoter function was observed for the
three main promoter haplotypes when tested in vitro.
Conclusion: Gene expression levels of the porcine PRKAG3 are associated with meat quality phenotypes relating to
glycolytic potential and IMF% in the Large White breed, while SNP variation in the promoter region of the gene is
associated with PRKAG3 gene expression and meat quality phenotypes.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia
663/664 - Aliments i nutrició. Enologia. Olis. Greixos
Pàgines
17
Publicat per
BMC
Publicat a
BMC Genetics
Citació
Ryan, M. F., Ruth M. Hamill, Aisling O’Halloran, Grace Davey, Jean McBryan, Anne Maria Mullen, Chris McGee, M. Gispert, O. I. Southwood, and T. Sweeney. 2012. “SNP Variation in the Promoter of the PRKAG3gene and Association With Meat Quality Traits in Pig.” BMC Genomic Data 13 (1): 66. doi:10.1186/1471-2156-13-66.
Programa
Qualitat i Tecnologia Alimentària
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