Phytogenics enhance welfare and vaccine efficacy against Vibrio anguillarum in European seabass (Dicentrarchus labrax) juveniles
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Autor/a
Montero, Daniel
Serradell, Antonio
Nedoluzhko, Artem
Fernández-Montero, Álvaro
Makol, Alex
Monzón-Atienza, Luis
Valdenegro, Victoria
Galindo-Villegas, Jorge
Acosta, Felix
Fecha de publicación
2024-02-24ISSN
0044-8486
Resumen
Disease prevention is pivotal in aquaculture, and while vaccines offer protective immunity, challenges such as
cost and low efficacy persist. The present study investigated the potential of plant-derived compounds, known as
phytogenics, to bolster the effectiveness of vaccines against vibriosis in European seabass. Two phytogenic
blends, namely PHYTO1 (terpenes) and PHYTO2 (terpenes and flavonoids) were supplemented to a commercial
diet to obtain three experimental diets: a non-supplemented control diet, PHYTO1 (a 200-ppm blend of garlic and
Lamiaceae oils with 87.5 mg kg− 1 terpenes), and PHYTO2 (a 1000 ppm blend containing citrus fruits, Asteraceae
and Lamiaceae oils with 57 mg kg− 1 terpenes and 55 mg kg− 1 flavonoids). Following vaccination by bath immersion, juvenile European seabass were divided into groups and fed one of the three diets for 30 days. After this
feeding period, fish were anesthetized and boosted with a single dose of vaccine through intraperitoneal injection. They continued to be fed their respective diets for another 30 days. At day 60, after the priming
vaccination, fish were challenged with Vibrio anguillarum via intraperitoneal injection. Various parameters were
measured at different time points post each vaccination, including total weight, circulating plasma cortisol and
glucose levels, serum immunoglobulin M (IgM) titers, antioxidant power of leucocytes, and the expression of
several antioxidant and immune-related genes. The results showed that fish fed with phytogenic supplements did
not differ in weight compared to the control group. However, they exhibited lower plasma cortisol and glucose
levels, increased IgM titers, and enhanced antioxidant protection and antioxidant power of head kidney leucocytes. In addition, phytogenics upregulated several immune-related genes in the gills and head kidney immediately after each vaccination. Notably, PHYTO2, enriched with flavonoids and terpenes, exhibited an even more
pronounced positive effect on boosted fish by reducing vaccine-associated stress while improving antioxidant
protection and modulating the vaccine-induced immune response. This synergistic effect of vaccination combined with phytogenics introduces new pathways for enhancing fish health in aquaculture.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
637 - Productos de los animales domésticos, de la caza y de la pesca
Páginas
11
Publicado por
Elsevier
Publicado en
Aquaculture
Citación
Montero, Daniel, Silvia Torrecillas, A. Serradell, Artem Nedoluzhko, Álvaro Fernández-Montero, Alex Makol, Luis Monzón-Atienza, et al. 2024. “Phytogenics Enhance Welfare and Vaccine Efficacy Against Vibrio Anguillarum in European Seabass (Dicentrarchus Labrax) Juveniles.” Aquaculture. 585: 740714. doi:10.1016/j.aquaculture.2024.740714.
Número del acuerdo de la subvención
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2016-79725-P/ES/Dieta funcional para sustitución de ingredientes marinos en peces: maximización de resistencia a patógenos vía refuerzo de barreras epiteliales y herramientas de inmunización/
Program
Aqüicultura
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