An In-Depth Study on the Inhibition of Quorum Sensing by Bacillus velezensis D-18: Its Significant Impact on Vibrio Biofilm Formation in Aquaculture
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Author
Monzón-Atienza, Luis
Bravo, Jimena
Gómez-Mercader, Antonio
Montero, Daniel
Ramos-Vivas, José
Galindo-Villegas, Jorge
Acosta, Félix
Publication date
2024-04-29ISSN
2076-2607
Abstract
Amid growing concerns about antibiotic resistance, innovative strategies are imperative in
addressing bacterial infections in aquaculture. Quorum quenching (QQ), the enzymatic inhibition of
quorum sensing (QS), has emerged as a promising solution. This study delves into the QQ capabilities
of the probiotic strain Bacillus velezensis D-18 and its products, particularly in Vibrio anguillarum 507
communication and biofilm formation. Chromobacterium violaceum MK was used as a biomarker in this
study, and the results confirmed that B. velezensis D-18 effectively inhibits QS. Further exploration into
the QQ mechanism revealed the presence of lactonase activity by B. velezensis D-18 that degraded both
long- and short-chain acyl homoserine lactones (AHLs). PCR analysis demonstrated the presence of a
homologous lactonase-producing gene, ytnP, in the genome of B. velezensis D-18. The study evaluated
the impact of B. velezensis D-18 on V. anguillarum 507 growth and biofilm formation. The probiotic not
only controls the biofilm formation of V. anguillarum but also significantly restrains pathogen growth.
Therefore, B. velezensis D-18 demonstrates substantial potential for preventing V. anguillarum diseases
in aquaculture through its QQ capacity. The ability to disrupt bacterial communication and control
biofilm formation positions B. velezensis D-18 as a promising eco-friendly alternative to conventional
antibiotics in managing bacterial diseases in aquaculture.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
637 - Produce of domestic (farmyard) animals and game
Pages
15
Publisher
MDPI
Is part of
Microorganisms
Citation
Monzón-Atienza, Luis, Jimena Bravo, Silvia Torrecillas, Antonio Gómez-Mercader, Daniel Montero, José Ramos-Vivas, Jorge Galindo-Villegas, and Félix Acosta. 2024. “An In-Depth Study on the Inhibition of Quorum Sensing by Bacillus velezensis D-18: Its Significant Impact on Vibrio Biofilm Formation in Aquaculture”. Microorganisms 12: 890. doi:10.3390/microorganisms12050890
Grant agreement number
EC/H2020/818367/EU/Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture/AquaIMPACT
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2590]
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