Differences in enteric pathogens and intestinal microbiota between diarrheic weaned piglets and healthy penmates
Visualitza/Obre
Data de publicació
2024-06-25ISSN
0378-1135
Resum
Postweaning diarrhea (PWD) is a multifactorial disease caused by different aetiological agents, like viruses or bacteria and where the role of the microbiota remains unclear. The aim of this study was to assess differences between healthy and diarrheic weaned pigs concerning the prevalence of pathogens and changes in the intestinal microbiota. Eighteen farms with PWD were selected and 277 fecal samples were collected (152 diarrheic vs 125 healthy). Presence of Rotavirus A (RVA), B (RVB), C (RVC) and Porcine Epidemic Diarrhea Virus (PEDV), virulence factors of Escherichia coli and Clostridioides difficile were analyzed by PCR. Finally, the microbiota composition was also study by 16 S rRNA sequencing on 148 samples (102 diarrheic vs 46 healthy). RVA (53.95 % vs 36 %, p=0.04) and RVB (49.67 % vs 28.8 %, p<0.001) were more frequent in diarrheic animals. Furthermore, RVA viral load was higher in diseased animals. VT2 toxin was significantly associated with diarrhea, whereas other virulence factors were not. Presence of C. difficile and PEDV was almost negligible. Regarding microbiota changes, Fusobacteriota phylum was more frequent in diarrheic samples and Ruminococcaceae family in healthy penmates. During the first week postweaning, Enterobacteriace and Campylobacteria were enriched in animals presenting diarrhea. Furthermore, Lactobacillus was detected in those individuals with no RVA infection. In conclusion, RVA seems to play a primary role in PWD. Classic E. coli virulence factors were not associated with diarrhea, indicating the need for revising their implication in disease. Moreover, Lactobacillus was found frequently in animals negative for RVA, suggesting some protective effect.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
8
Publicat per
Elsevier
Publicat a
Veterinary Microbiology
Citació
Garcias, Biel, Lourdes Migura-Garcia, Noemí Giler, Marga Martín, and Laila Darwich. “Differences in Enteric Pathogens and Intestinal Microbiota Between Diarrheic Weaned Piglets and Healthy Penmates.” Veterinary Microbiology 295 (August 1, 2024): 110162. doi:10.1016/j.vetmic.2024.110162.
Número de l'acord de la subvenció
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/ES/OPTIMIZATION OF DIAGNOSIS AND TREATMENT OF POSTWEANING DIARRHEAS IN PIGS: TOWARDS A RATIONAL USE OF ANTIBIOTICS/
Programa
Sanitat Animal
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