Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
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Autor/a
Gil-Villalba, Sergio
Palau, Jordi
Soder-Walz, Jesica M.
Vallecillo, Miguel A.
Corregidor, Jordi
Tirado, Andrea
Shouakar-Stash, Orfan
Soler, Albert
Rosell, Monica
Fecha de publicación
2024-08-14ISSN
0048-9697
Resumen
Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate the CEs (PCE, TCE and cis-DCE) bioremediation using EVO in a contaminated site. A multiple approach using C and Cl-CSIA, quantification of Dehalococcoides (Dhc) and specific reductive dechlorination (RD) gene population, and hydrochemical data in microcosm experiments and field samples was applied. Despite the high partitioning of CEs into the EVO phase, the carbon isotopic values of the remaining CEs fraction in the aqueous phase did not exhibit significant changes caused by phase partitioning in laboratory experiments. Both microcosm experiments and field data revealed a rapid RD of PCE and TCE, resulting in the transient accumulation of cis-DCE, which was slowly degraded to vinyl chloride (VC). These results agreed with the presence of Dhc populations and a shift to stronger reducing conditions in the field: i) RD functional genes (tceA, vcrA and bvcA) exhibited a trend to higher values and ii) a substantial increase in Dhc populations (up to 30% of the total bacterial populations) was observed over time. The dual-element isotope slope ΛC-Cl for RD of cis-DCE obtained from field data (ΛC – Cl = 5 ± 3) was similar to the one determined from the microcosm experiments under controlled anoxic conditions (ΛC – Cl = 4.9 ± 0.8). However, ΛC-Cl values differ from those reported so far for laboratory studies with Dhc strains and mixed cultures containing Dhc, i.e., between 8.3 and 17.8. This observation underscores the potential variety of reductive dehalogenases involved during cis-DCE RD and the importance of determining site-specific Λ and ɛ values in order to improve the identification and quantification of transformation processes in the field.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
631 - Agricultura. Agronomía. Maquinaria agrícola. Suelos. Edafología agrícola
Páginas
12
Publicado por
Elsevier
Publicado en
Science of the Total Environment
Citación
Gil-Villalba, Sergio, Jordi Palau, Jesica M Soder-Walz, Miguel A Vallecillo, Jordi Corregidor, Andrea Tirado, Orfan Shouakar-Stash, et al. 2024. “Use of Isotopic (C, Cl) and Molecular Biology Tools to Assess Biodegradation in a Source Area of Chlorinated Ethenes after Biostimulation with Emulsified Vegetable Oil (EVO).” The Science of the Total Environment 951. doi:10.1016/j.scitotenv.2024.175351.
Número del acuerdo de la subvención
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/CGL2017-87216-C4-1-R/ES/PROCESOS DE ATENUACION DE CONTAMINANTES CONVENCIONALES Y EMERGENTES PROCEDENTES DE FUENTES AGRICOLAS E INDUSTRIALES EN AGUAS/PACE-ISOTEC
FEDER/ / /EU/ /
Program
Sostenibilitat en Biosistemes
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