Diversity of Multi-Drug Resistant Avian Pathogenic Escherichia coli (APEC) Causing Outbreaks of Colibacillosis in Broilers during 2012 in Spain
Visualitza/Obre
Autor/a
Solà-Ginés, Marc
Cameron-Veas, Karla
Dolz, Roser
Dahbi, Ghizlane
Viso, Susana
Mora, Azucena
Blanco, Jorge
Piedra-Carrasco, Nuria
González-López, Juan José
Andón Mañero, Miquel
Data de publicació
2015-11-23ISSN
1932-6203
Resum
Avian pathogenic Escherichia coli (APEC) are the major cause of colibacillosis in poultry production. In this study, a total of 22 E. coli isolated from colibacillosis field cases and 10 avian faecal E. coli (AFEC) were analysed. All strains were characterised phenotypically by susceptibility testing and molecular typing methods such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The presence of 29 virulence genes associated to APEC and human extraintestinal pathogenic E. coli (ExPEC) was also evaluated. For cephalosporin resistant isolates, cephalosporin resistance genes, plasmid location and replicon typing was assessed. Avian isolates belonged to 26 O:H serotypes and 24
sequence types. Out of 22 APEC isolates, 91% contained the virulence genes predictors of APEC; iutA, hlyF, iss, iroN and ompT. Of all strains, 34% were considered ExPEC. PFGE analysis demonstrated a high degree of genetic polymorphism. All strains were multi-resistant, including those isolated from healthy animals. Eleven strains were resistant to cephalosporins; six contained blaCTX-M-14, two blaSHV-12, two blaCMY-2 and one blaSHV-2. Two strains harboured qnrA, and two qnrA together with aac(6’)-Ib-cr. Additionally, the emergent clone O25b:H4-B2-ST131 was isolated from a healthy animal which harboured blaCMY-2 and qnrS genes. Cephalosporin resistant genes were mainly associated to the presence of IncK replicons. This study demonstrates a very diverse population of multi-drug resistant E. coli containing a high number of virulent genes. The E. coli population among broilers is a reservoir
of resistance and virulence-associated genes that could be transmitted into the community through the food chain. More epidemiological studies are necessary to identify clonal groups and resistance mechanisms with potential relevance to public health.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
619 - Veterinària
Pàgines
14
Publicat per
Public Library of Science
Publicat a
PLoS ONE
Citació
Solà-Ginés, Marc, Karla Cameron-Veas, Ignacio Badiola, Roser Dolz, Natalia Majó, Ghizlane Dahbi, and Susana Viso et al. 2015. "Diversity Of Multi-Drug Resistant Avian Pathogenic Escherichia Coli (APEC) Causing Outbreaks Of Colibacillosis In Broilers During 2012 In Spain". PLOS ONE 10 (11): e0143191. doi:10.1371/journal.pone.0143191.
Número de l'acord de la subvenció
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011-28836/ES/EVALUACION DE CONSUMOS DE ANTIMICROBIANOS COMO FACTORES DE RIESGO RELACIONADOS CON LA APARICION DE RESISTENCIA A CEFALOSPORINAS EN ANIMALES DESTINADOS AL CONSUMO/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2013-47852-R/ES/FUENTES DE TRANSMISION DEL GRUPO CLONAL PANDEMICO ST131 DE ESCHERICHIA COLI. CARACTERIZACION MOLECULAR DEL PATOGENO Y DE SU VIRULENCIA IN VIVO, EVALUACION DE RIESGO Y CONTROL/
FEDER/ / /EU/ /
Programa
Sanitat Animal
Aquest element apareix en la col·lecció o col·leccions següent(s)
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