The NS segment of H5N1 avian influenza viruses (AIV) enhances the virulence of an H7N1 AIV in chickens
View/Open
Author
Vergara-Alert, Júlia
Chaves, Aida J
Rivas, Raquel
Dolz, Roser
Wang, Zhongfang
Pleschka, Stephan
Publication date
2014-01-25ISSN
0928-4249
Abstract
Some outbreaks involving highly pathogenic avian influenza viruses (HPAIV) of subtypes H5 and H7 were caused by avian-to-human transmissions. In nature, different influenza A viruses can reassort leading to new viruses with new characteristics. We decided to investigate the impact that the NS-segment of H5 HPAIV would have on viral pathogenicity of a classical avian H7 HPAIV in poultry, a natural host. We focussed this study based on our previous work that demonstrated that single reassortment of the NS-segment from an H5 HPAIV into an H7 HPAIV changes the ability of the virus to replicate in mammalian hosts. Our present data show that two different H7-viruses containing an NS-segment from H5–types (FPV NS GD or FPV NS VN) show an overall highly pathogenic phenotype compared with the wild type H7–virus (FPV), as characterized by higher viral shedding and earlier manifestation of clinical signs. Correlating with the latter, higher amounts of IFN-β mRNA were detected in the blood of NS-reassortant infected birds, 48 h post-infection (pi). Although lymphopenia was detected in chickens from all IVinfected groups, also 48 h pi those animals challenged with NS-reassortant viruses showed an increase of peripheral monocyte/macrophage-like cells expressing high levels of IL-1β, as determined by flow cytometry. Taken together, these findings highlight the importance of the NS-segment in viral pathogenicity which is directly involved in triggering antiviral and pro-inflammatory cytokines found during HPAIV pathogenesis in chickens.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
575 - General genetics. General cytogenetics
619 - Veterinary science
Pages
11
Publisher
BMC
Is part of
Veterinary Research
Citation
Vergara-Alert, Júlia, Núria Busquets, Maria Ballester, Aida J Chaves, Raquel Rivas, Roser Dolz, and Zhongfang Wang et al. 2014. "The NS Segment Of H5N1 Avian Influenza Viruses (AIV) Enhances The Virulence Of An H7N1 AIV In Chickens". Veterinary Research 45 (1): 7. doi:10.1186/1297-9716-45-7.
Grant agreement number
MEC/ /AGL2007-60434/ES/PAPEL DE LA INMUNIDAD INNATA DEL HOSPEDADOR EN LA PROTECCION Y PATOGENIA DE LA INFECCION POR EL VIRUS DE INFLUENZA AVIAR/GAN
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229 -C03-01/ES/CARACTERIZACION DE MECANISMOS IMPLICADOS EN PROTECCION FRENTE AL VIRUS DE LA PESTE PORCINA AFRICANA: DESARROLLO DE VACUNA FRENTE AL VPPA/
Program
Genètica i Millora Animal
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2850]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/