Souffle/Spastizin Controls Secretory Vesicle Maturation during Zebrafish Oogenesis
Visualitza/Obre
Autor/a
Kanagaraj, Palsamy
Gautier-Stein, Amandine
Riedel, Dietmar
Schomburg, Christoph
Vollack, Nadine
Dosch, Roland
Data de publicació
2014-06-26ISSN
1553-7390
Resum
During oogenesis, the egg prepares for fertilization and early embryogenesis. As a consequence, vesicle transport is very active during vitellogenesis, and oocytes are an outstanding system to study regulators of membrane trafficking. Here, we combine zebrafish genetics and the oocyte model to identify the molecular lesion underlying the zebrafish souffle (suf) mutation. We demonstrate that suf encodes the homolog of the Hereditary Spastic Paraplegia (HSP) gene SPASTIZIN (SPG15). We show that in zebrafish oocytes suf mutants accumulate Rab11b-positive vesicles, but trafficking of recycling endosomes is not affected. Instead, we detect Suf/Spastizin on cortical granules, which undergo regulated secretion. We demonstrate genetically that Suf is essential for granule maturation into secretion competent dense-core vesicles describing a novel role for Suf in vesicle maturation. Interestingly, in suf mutants immature, secretory precursors accumulate, because they fail to pinch-off Clathrin-coated buds. Moreover, pharmacological inhibition of the abscission regulator Dynamin leads to an accumulation of immature secretory granules and mimics the suf phenotype. Our results identify a novel regulator of
secretory vesicle formation in the zebrafish oocyte. In addition, we describe an uncharacterized cellular mechanism for Suf/Spastizin activity during secretion, which raises the possibility of novel therapeutic avenues for HSP research.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia
639 - Caça. Pesca. Piscicultura
Pàgines
17
Publicat per
Public Library of Science
Publicat a
PLoS Genetics
Citació
Kanagaraj, Palsamy, Amandine Gautier-Stein, Dietmar Riedel, Christoph Schomburg, Joan Cerdà, Nadine Vollack, and Roland Dosch. 2014. "Souffle/Spastizin Controls Secretory Vesicle Maturation During Zebrafish Oogenesis". Plos Genetics 10 (6): e1004449. doi:10.1371/journal.pgen.1004449.
Programa
Aqüicultura
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