Mapping and Introgression of QTL Involved in Fruit Shape Transgressive Segregation into ‘Piel de Sapo’ Melon (Cucucumis melo L.)
Ver/Abrir
Autor/a
DÍaz, Aurora
Zarouri, Belkacem
Fergany, Mohamed
Álvarez, José M.
Picó, Belén
Monforte, Antonio J.
Fecha de publicación
2014-08-15ISSN
1932-6203
Resumen
A mapping F2 population from the cross ‘Piel de Sapo’6PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de
Sapo’ background which yields round melons.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
575 - Genética general. Citogenética general. Inmunogenética. Evolución. Filogenia
633 - Cultivos y producciones
Páginas
12
Publicado por
Public Library of Science
Publicado en
PLoS ONE
Citación
Díaz, Aurora, Belkacem Zarouri, Mohamed Fergany, Iban Eduardo, José M. Álvarez, Belén Picó, and Antonio J. Monforte. 2014. "Mapping And Introgression Of QTL Involved In Fruit Shape Transgressive Segregation Into ‘Piel De Sapo’ Melon (Cucucumis Melo L.)". Plos ONE 9 (8): e104188. doi:10.1371/journal.pone.0104188.
Número del acuerdo de la subvención
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2009-12698-C02-02/ES/El Control Genetico De La Forma Del Fruto Y Domesticacion Del Melon En Varias Fuentes De Germoplasma/
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40130-C02-02/ES/DESCIFRANDO LA BASE GENETICA DE LA MORFOLOGIA DEL FRUTO Y LA DOMESTICACION DE MELON/
Program
Genòmica i Biotecnologia
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