Screening culture filtrates of fungi for activity against Tylenchulus semipenetrans
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Publication date
2009-12-01ISSN
1695-971X
Abstract
Culture filtrates of 20 fungi isolated from citrus soils were screened for their activity against Tylenchulus
semipenetrans in both in vitro and greenhouse tests. The filtrates of Talaromyces cyanescens (isolates 2-4 and 2-5),
Paecilomyces lilacinus, Chaetomium robustum, Acremonium strictum, Engyodontium album, Myrothecium verrucaria,
Emericella rugulosa, and Tarracomyces gigaspora consistently inhibited the motility of second-stage juveniles at
various concentrations of the filtrate. Dose-response models were used to determine the filtrate concentration required
to inhibit the motility of 50% of the juveniles (CI50). The culture filtrate of P. lilacinus showed the highest activity
with a CI50 value of 58% that differed from that of C. robustum (CI50 = 68%), and A. strictum CI50 = 82%. The culture
filtrates of P. lilacinus, E. album, and T. cyanescens 2-5 maintained their activity when autoclaved at 120ºC for 20
min. The autoclaved filtrate of T. cyanescens 2-4 was more effective at inhibiting juvenile motility (CI50 = 28%) than
that of T. cyanescens 2-5 (CI50 = 80%), C. robustum (CI50 = 72 %) and P. lilacinus (CI50 = 72%). The culture filtrate
of T. cyanescens 2-4 also inhibited egg hatching. Nematode reproduction on Cleopatra mandarin and Carrizo citrange
were respectively reduced by the culture filtrate of P. lilacinus and the autoclaved filtrate of T. cyanescens 2-4. These
results support the hypothesis that soil fungi may contribute to regulate nematode densities by the production of
secondary metabolites with nematicidal activity.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
632 - Plant damage, injuries. Plant diseases. Pests, organisms injurious to plants. Plant protection
Pages
9
Publisher
Consejo Superior de Investigaciones Científicas
Is part of
Spanish Journal of Agricultural Research
Citation
Lucas, S. Verdejo, A. Viera, A.M. Stchigel, and F.J. Sorribas. 2009. “Screening Culture Filtrates of Fungi for Activity Against Tylenchulus Semipenetrans.” Spanish Journal of Agricultural Research 7 (4): 896-904. doi: 10.5424/sjar/2009074-1103
Grant agreement number
INIA/Programa Nacional de Recursos y Tecnologías Agroalimentarias/RTA2005-00086-00-00/ES/Control de Tylenchulus semipenetrans mediante métodos biológicos, genéticos y culturales compatilbes con la producción integrada de cítricos/
Program
Protecció Vegetal Sostenible
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2811]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/