Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells
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Autor/a
Sanchez-Henao, Andres
Mandalakis, Manolis
Peristeraki, Panagiota
Molgó, Jordi
Sureda, Francesc X.
Data de publicació
2024-08-05ISSN
0021-8561
Resum
Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC–MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
574 - Ecologia general i biodiversitat
Pàgines
26
Publicat per
American Chemical Society
Publicat a
Journal of Agricultural and Food Chemistry
Citació
Reverté, Jaume, Maria Rambla-Alegre, Andres Sanchez-Henao, Manolis Mandalakis, Panagiota Peristeraki, Jordi Molgó, Jorge Diogène, Francesc X. Sureda, and Mònica Campàs. 2024. “Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined With Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells.” Journal of Agricultural and Food Chemistry 72 (32): 18192–200. https://doi.org/10.1021/acs.jafc.4c04321.
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MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
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Aigües Marines i Continentals
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