Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells
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Author
Sanchez-Henao, Andres
Mandalakis, Manolis
Peristeraki, Panagiota
Molgó, Jordi
Sureda, Francesc X.
Publication date
2024-08-05ISSN
0021-8561
Abstract
Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC–MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
26
Publisher
American Chemical Society
Is part of
Journal of Agricultural and Food Chemistry
Citation
Reverté, Jaume, Maria Rambla-Alegre, Andres Sanchez-Henao, Manolis Mandalakis, Panagiota Peristeraki, Jordi Molgó, Jorge Diogène, Francesc X. Sureda, and Mònica Campàs. 2024. “Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined With Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells.” Journal of Agricultural and Food Chemistry 72 (32): 18192–200. https://doi.org/10.1021/acs.jafc.4c04321.
Grant agreement number
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2850]
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