Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined with Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells
Ver/Abrir
Este documento contiene ficheros embargados hasta el dia 05-08-2025
Autor/a
Sanchez-Henao, Andres
Mandalakis, Manolis
Peristeraki, Panagiota
Molgó, Jordi
Sureda, Francesc X.
Fecha de publicación
2024-08-05ISSN
0021-8561
Resumen
Tetrodotoxin (TTX) is a potent marine neurotoxin, responsible for numerous poisoning incidents and some human fatalities. To date, more than 30 TTX analogues have been identified, but their individual toxicities and roles in poisoning remain largely unknown. In this work, the toxicity equivalency factors (TEFs) of five TTX analogues were determined by assessing the blockade of voltage-gated sodium channels in Neuro-2a cells using automated patch clamp (APC). All TTX analogues were less toxic than TTX. The derived TEFs were applied to the individual TTX analogues concentrations measured in pufferfish samples, using liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). A comparison of these results with those obtained from APC analysis demonstrated that TEFs can be effectively used to translate LC–MS/MS analytical data into meaningful toxicological information. This is the first study to utilize APC device for the toxicological assessment of TTX analogues, highlighting its potential as a bioanalytical tool for seafood safety management and human health protection.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
574 - Ecología general y biodiversidad
Páginas
26
Publicado por
American Chemical Society
Publicado en
Journal of Agricultural and Food Chemistry
Citación
Reverté, Jaume, Maria Rambla-Alegre, Andres Sanchez-Henao, Manolis Mandalakis, Panagiota Peristeraki, Jordi Molgó, Jorge Diogène, Francesc X. Sureda, and Mònica Campàs. 2024. “Toxicity Equivalency Factors for Tetrodotoxin Analogues Determined With Automated Patch Clamp on Voltage-Gated Sodium Channels in Neuro-2a Cells.” Journal of Agricultural and Food Chemistry 72 (32): 18192–200. https://doi.org/10.1021/acs.jafc.4c04321.
Número del acuerdo de la subvención
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
Program
Aigües Marines i Continentals
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2850]
Derechos
Copyright © 2024 American Chemical Society