Detection of tetrodotoxins in juvenile pufferfish Lagocephalus sceleratus (Gmelin, 1789) from the North Aegean Sea (Greece) by an electrochemical magnetic bead-based immunosensing tool
Visualitza/Obre
Autor/a
Leonardo, Sandra
Kiparissis, Sotiris
Rambla-Alegre, Maria
Almarza, Sergi
Andree, Karl B.
Christidis, Aris
Flores, Cintia
Caixach, Josep
Campbell, Katrina
Elliott, Christopher T.
Aligizaki, Katerina
Data de publicació
2019-03-29ISSN
0308-8146
Resum
Two small Lagocephalus sceleratus juveniles were captured in picarel targeting catches from North Aegean Sea (Greece) in the autumn of 2017. An electrochemical immunosensing tool using magnetic beads as immobilisation support was developed and applied to the rapid screening of tetrodotoxins (TTXs), potent neurotoxins that constitute a food safety hazard when present in seafood. This tool revealed the presence of TTXs in both individuals. Results were compared with those provided by mELISA and LC-HRMS, the latter confirming the presence of TTX. Some of the tissues contained TTX contents close to or above 2 mg/kg. L. sceleratus juveniles had been considered as non-toxic and, to our knowledge, this is the first report of high TTX levels in small L. sceleratus individuals. Such specimens can be mistaken with other edible species, posing a threat to consumers. The availability of low-cost and user-friendly tools for TTXs detection will contribute to guarantee seafood safety.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
63 - Agricultura. Silvicultura. Zootècnia. Caça. Pesca
Pàgines
31
Publicat per
Elsevier
Publicat a
Food Chemistry
Citació
Leonardo, Sandra, Sotiris Kiparissis, Maria Rambla-Alegre, Sergi Almarza, Ana Roque, Karl B. Andree, and Aris Christidis et al. 2019. "Detection Of Tetrodotoxins In Juvenile Pufferfish Lagocephalus Sceleratus (Gmelin, 1789) From The North Aegean Sea (Greece) By An Electrochemical Magnetic Bead-Based Immunosensing Tool". Food Chemistry. Elsevier BV. doi:10.1016/j.foodchem.2019.03.148.
Número de l'acord de la subvenció
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/DISPOSITIVOS BIOANALITICOS PARA LA DETECCION RAPIDA Y RENTABLE DE GAMBIERDISCUS Y CIGUATOXINAS/CIGUASENSING
Programa
Aigües Marines i Continentals
Aqüicultura
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