Gene expression analysis of the innate immune system during early rearing and weaning of meagre (Argyrosomus regius)
Ver/Abrir
Autor/a
Campoverde, Cindy
Milne, Douglas J.
Secombes, Christopher J.
Andree, Karl B.
Fecha de publicación
2019-10-06ISSN
1050-4648
Resumen
The present study is the first report of some representative innate immune genes in meagre (Argyrosomus regius) larvae. This study has specifically focused on the growth period from hatching to the juvenile stage, a critical time in marine fish development when reliance on innate immune mechanisms are required for survival. We report molecular cloning of partial open reading frames and expression patterns for some innate immune genes (c3, cox2, met, lyzc, mxp, myd88, nod2, nod3). In addition, phylogenetic analyses of some of the sequences obtained was performed where confusion among closely allied isoforms may have existed. These results show the met isoform from meagre is met II, an isoform more similar to a homolog described in Larimichthys crocea; lysozyme (lyzc) corresponds to the c-type and NOD isoforms (nod2, nod3) separate into different clades confirming their distinctness within a common evolutionary history. Gene expression profiles of innate genes were investigated, for nine developmental stages, from 8 days post-hatching (dph) to 120 dph. Present results demonstrated that c3, cox2, met II, lyzc, mxp, myd88, nod2, and nod3 were expressed in all stages of larval development and displayed distinct expression profiles in separate tissues (kidney, spleen gut and gill). Moreover, expression patterns suggested theses innate immune genes may be influenced by feeding practices, i.e. switching from live prey (rotifer and Artemia) and weaning onto an inert commercial diet. In addition to evaluating changes in gene expression during early development, this study evaluated the modulation of gene expression by means of in vivo trials in juveniles that were stimulated with PAMPs (LPS, poly I:C, β-glucan). These results revealed significant changes in mRNA levels of target genes in the kidney, spleen, gut and gills. However, expression profiles differed in magnitude depending on the stimulant and/or tissue. These results are discussed in terms of their relevance and potential application in aquaculture practices.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
639 - Caza. Pesca. Piscicultura
Páginas
36
Publicado por
Elsevier
Publicado en
Fish and Shellfish Immunology
Citación
Campoverde, Cindy, Douglas J. Milne, Christopher J. Secombes, Alicia Estévez, Enric Gisbert, and Karl B. Andree. 2019. "Gene Expression Analysis Of The Innate Immune System During Early Rearing And Weaning Of Meagre (Argyrosomus Regius)". Fish & Shellfish Immunology 94: 819-832. Elsevier BV. doi:10.1016/j.fsi.2019.10.009.
Número del acuerdo de la subvención
EC/FP7/603121/EU/Exploring the biological and socio-economic potential of new-emerging candidate fish species for the expansion of the European aquaculture industry/DIVERSIFY
Program
Aqüicultura
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2831]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by-nc-nd/4.0/