High prevalence and diversity of extended-spectrum β-lactamase and emergence of OXA-48 producing Enterobacterales in wildlife in Catalonia
Ver/Abrir
Autor/a
Darwich, Laila
Vidal, Anna
Seminati, Chiara
Albamonte, Andreu
Casado, Alba
López, Ferrán
Molina-López, Rafael A.
Migura-Garcia, Lourdes
Fecha de publicación
2019-08-05ISSN
1932-6203
Resumen
Most of the studies focused on antimicrobial resistance (AMR) performed in wildlife describe
Escherichia coli as the principal indicator of the selective pressure. In the present study, several species of Enterobacterales with a large panel of cephalosporin resistant (CR) genes
have been isolated from wildlife in Catalonia. A total of 307 wild animals were examined to
determine the prevalence of CR enterobacteria, AMR phenotypes and the presence of common carbapenem and CR genes. The overall prevalence of CR-phenotype was 13% (40/
307): 17.3% in wild mammals (18/104) and 11.5% in wild birds (22/191) (p<0.01). Hedgehogs showed the highest prevalence (13.5% of 104) of the mammal specimens, and raptors
the highest in bird specimen (7.3% of 191). Although CR E. coli was the most frequently isolated (45%), other CR- Enterobacterales like Klebsiella pneumoniae (20%), Citrobacter
freundii (15%), Enterobacter cloacae (5%), Proteus mirabilis (5%), Providencia spp (5%)
and Serratia marcescens (2.5%) were also isolated. A high diversity of CR genes was identified among the isolates, with 50% yielding blaCMY-2, 23% blaSHV-12, 20% blaCMY-1 and
18% blaCTX-M-15. Additionally, resistance to carbapenems associated to OXA-48 gene
was found. Most of the CR isolates, principally K. pneumoniae and C. freundii, were multiresistant with co-resistance to fluoroquinolones, tetracycline, sulphonamides and aminoglycosides. This study reports high prevalence of Enterobacterales harbouring a variety of CR
genes and OXA-48 mediated-carbapenem resistance, all of them frequently associated to
nosocomial human infections, for the first time in wild mammals and wild birds. Implementation of control measures to reduce the impact of anthropogenic pressure in the environment
is urgently needed.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
15
Publicado por
Public Library of Science
Publicado en
PLoS ONE
Citación
Darwich, Laila, Anna Vidal, Chiara Seminati, Andreu Albamonte, Alba Casado, Ferrán López, Rafael A. Molina-López, and Lourdes Migura-Garcia. 2019. "High Prevalence And Diversity Of Extended-Spectrum Β-Lactamase And Emergence Of OXA-48 Producing Enterobacterales In Wildlife In Catalonia". PLOS ONE 14 (8): e0210686. Public Library of Science (PLoS). doi:10.1371/journal.pone.0210686.
Program
Sanitat Animal
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