Analysis of porcine IGF2 gene expression in adipose tissue and its effect on fatty acid composition
Ver/Abrir
Autor/a
Criado-Mesas, Lourdes
Ballester, Maria
Castelló, Anna
Benítez, Rita
Fernández, Ana Isabel
Folch, Josep M.
Fecha de publicación
2019-08-08ISSN
1932-6203
Resumen
IGF2:g.3072G>A polymorphism has been described as the causal mutation of a maternally imprinted QTL for muscle growth and fat deposition in pigs. The objective of the current work was to study the association between the IGF2:g.3072G>A polymorphism and the IGF2 gene expression and its effect on fatty acid composition in adipose tissue in different pig genetic backgrounds. A cis-eQTL region associated with the IGF2 mRNA expression in adipose tissue was identified in an eGWAS with 355 animals. The IGF2 gene was located in this genomic interval and IGF2g.3072G>A was the most significant SNP, explaining a 25% of the gene expression variance. Significant associations between IGF2:g.3072G>A polymorphism and oleic (C18:1(n-9); p-value = 4.18x10-07), hexadecanoic (C16:1(n-9); p-value = 4.04x10-07), linoleic (C18:2(n-6); p-value = 6.44x10-09), α-linoleic (C18:3(n-3); p-value = 3.30x10-06), arachidonic (C20:4(n-6); p-value = 9.82x10-08) FAs and the MUFA/PUFA ratio (p-value = 2.51x10-9) measured in backfat were identified. Animals carrying the A allele showed an increase in IGF2 gene expression and higher PUFA and lower MUFA content. However, in additional studies was observed that there could be other proximal genetic variants affecting FA composition in adipose tissue. Finally, no differences in the IGF2 gene expression in adipose tissue were found between heterozygous animals classified according to the IGF2:g.3072G>A allele inherited from the father (APGM or AMGP). However, pyrosequencing analysis revealed that there is imprinting of the IGF2 gene in muscle and adipose tissues, with stronger differences among the paternally and maternally inherited alleles in muscle. Our results suggested that IGF2:g.3072G>A polymorphism plays an important role in the regulation of IGF2 gene expression and can be involved in the fatty acid composition in adipose tissue. In both cases, further studies are still needed to deepen the mechanism of regulation of IGF2 gene expression in adipose tissue and the IGF2 role in FA composition.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
636 - Explotación y cría de animales. Cría del ganado y de animales domésticos
Páginas
18
Publicado por
Public Library of Science
Publicado en
Plos One
Citación
Criado-Mesas, Lourdes, Maria Ballester, Daniel Crespo-Piazuelo, Anna Castelló, Rita Benítez, Ana Isabel Fernández, and Josep M. Folch. 2019. "Analysis Of Porcine IGF2 Gene Expression In Adipose Tissue And Its Effect On Fatty Acid Composition". PLOS ONE 14 (8): e0220708. Public Library of Science (PLoS). doi:10.1371/journal.pone.0220708.
Número del acuerdo de la subvención
MINECO-FEDER/Programa estatal de I+D+I orientada a los retos de la sociedad/AGL2014-56369-C2-2-R/ES//Validación genética y funcional de QTLS, genes y redes génicas y estudio del efecto del microbioma sobre crecimiento, deposición de grasa y calidad de carne en porcino. S2/
MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-82641-R/ES/Genómica funcional, biología de sistemas y microbiomica aplicadas a la identificación de reguladores genéticos del crecimiento, engrasamiento y calidad de la carne en porcino/
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC‑2013‑12573/ES/ /
Program
Genètica i Millora Animal
Este ítem aparece en la(s) siguiente(s) colección(ones)
- ARTICLES CIENTÍFICS [2831]
Excepto si se señala otra cosa, la licencia del ítem se describe como http://creativecommons.org/licenses/by/4.0/