Role of AMPK signalling pathway during compensatory growth in pigs
Visualitza/Obre
Autor/a
Ballester, Maria
Amills, Marcel
González-Rodríguez, Olga
Cardoso, Tainã F.
González-Prendes, Rayner
Panella-Riera, Núria
Díaz, Isabel
Tibau, Joan
Data de publicació
2018-09-17ISSN
1471-2164
Resum
Background: The molecular basis of compensatory growth in monogastric animals has not yet been fully explored.
Herewith, in this study we aim to determine changes in the pig skeletal muscle transcriptome profile during
compensatory growth following a feed restriction period. A RNA-Seq experiment was performed with a total of 24
females belonging to a Duroc commercial line. Half of the animals received either a restricted (RE) or ad libitum
(AL) diet during the first fattening period (60–125 d of age). After that, all gilts were fed ad libitum for a further ~30
d until the age of ~155 d, when animals were slaughtered and samples of gluteus medius muscle were harvested to
perform RNA-Seq analyses and intramuscular fat content determination.
Results: During the period following food restriction, RE animals re-fed ad libitum displayed compensatory growth,
showed better feed conversion rate and tended to deposit more subcutaneous fat than AL fed animals. Animals
were slaughtered in the phase of accelerated growth, when RE animals had not completely compensated the
performance of AL group, showing lower live and carcass weights. At intramuscular level, RE gilts showed a higher
content of polyunsaturated fatty acids during the compensatory growth phase. The comparison of RE and AL
expression profiles allowed the identification of 86 (ǀlog2Fold-Changeǀ > 1, padj < 0.05) differentially expressed (DE)
genes. A functional categorization of these DE genes identified AMPK Signaling as the most significantly enriched
canonical pathway. This kinase plays a key role in the maintenance of energy homeostasis as well as in the
activation of autophagy. Among the DE genes identified as components of AMPK Signaling pathway, five out of six
genes were downregulated in RE pigs.
Conclusions: Animals re-fed after a restriction period exhibited a less oxidative metabolic profile and catabolic
processes in muscle than animals fed ad libitum. The downregulation of autophagy observed in the skeletal muscle
of pigs undergoing compensatory growth may constitute a mechanism to increase muscle mass thus ensuring an
accelerated growth rate. These results reveal that the downregulation of AMPK Signaling plays an important role in
compensatory growth in pigs.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
575 - Genètica general. Citogenètica general. Immunogenètica. Evolució. Filogènia
Pàgines
12
Publicat per
BioMed Central
Publicat a
BMC Genomics
Citació
Ballester, M., Amills, M., González-Rodríguez, O., Cardoso, T., Pascual, M., & González-Prendes, R. et al. (2018). Role of AMPK signalling pathway during compensatory growth in pigs. BMC Genomics, 19(1). doi:10.1186/s12864-018-5071-5
Número de l'acord de la subvenció
MINECO/AGL2013/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
Programa
Genètica i Millora Animal
Qualitat i Tecnologia Alimentària
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