A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post–vaccination monitoring
Ver/Abrir
Autor/a
Wang, Lihua
Mi, Shijiang
Madera, Rachel
Borca, Manuel V.
Ren, Jingqiang
Cunningham, Chase
Cino-Ozuna, Ada G.
Li, Hongwei
Tu, Changchun
Gong, Wenjie
Shi, Jishu
Fecha de publicación
2020-01-14ISSN
1746-6148
Resumen
Background: Virus neutralization test (VNT) is widely used for serological survey of classical swine fever (CSF) and efficacy evaluation of CSF vaccines. However, VNT is a time consuming procedure that requires cell culture and live virus manipulation. C-strain CSF vaccine is the most frequently used vaccine for CSF control and prevention. In this study, we presented a neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with the emphasis on the replacement of VNT for C-strain post–vaccination monitoring. Results: One monoclonal antibody (6B211) which has potent neutralizing activity against C-strain was generated. A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. By testing C-strain VNT negative pig sera (n = 445) and C-strain VNT positive pig sera (n = 70), the 6B211 based cELISA showed 100% sensitivity (95% confidence interval: 94.87 to 100%) and 100% specificity (95% confidence interval: 100 to 100%). The C-strain antibody can be tested in pigs as early as 7 days post vaccination with the cELISA. By testing pig sera (n = 139) in parallel, the cELISA showed excellent agreement (Kappa = 0.957) with VNT. The inhibition rate of serum samples in the cELISA is highly correlated with their titers in VNT (r2 = 0.903, p < 0.001). In addition, intra- and inter-assays of the cELISA exhibited acceptable repeatability with low coefficient of variations (CVs). Conclusions: This novel cELISA demonstrated excellent agreement and high level correlation with VNT. It is a reliable tool for sero-monitoring of C-strain vaccination campaign because it is a rapid, simple, safe and cost effective assay that can be used to monitor vaccination-induced immune response at the population level.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
619 - Veterinaria
Páginas
8
Publicado por
BMC
Publicado en
BMC Veterinary Research
Citación
Wang, Lihua, Shijiang Mi, Rachel Madera, Llilianne Ganges, Manuel V. Borca, Jingqiang Ren, and Chase Cunningham et al. 2020. "A Neutralizing Monoclonal Antibody-Based Competitive ELISA For Classical Swine Fever C-Strain Post–Vaccination Monitoring". BMC Veterinary Research 16 (1). doi:10.1186/s12917-020-2237-6.
Program
Sanitat Animal
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