Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey
View/Open
Author
Boumaiza, Mohamed
Colarusso, Andrea
Parrilli, Ermenegilda
Casillo, Angela
Corsaro, Maria Michela
Picone, Delia
Leone, Serena
Tutino, Maria Luisa
Publication date
2018-08-15ISSN
1475-2859
Abstract
Background
Recent biotechnological advancements have allowed for the adoption of Lactococcus lactis, a typical component of starter cultures used in food industry, as the host for the production of food-grade recombinant targets. Among several advantages, L. lactis has the important feature of growing on lactose, the main carbohydrate in milk and a majoritarian component of dairy wastes, such as cheese whey.
Results
We have used recombinant L. lactis NZ9000 carrying the nisin inducible pNZ8148 vector to produce MNEI, a small sweet protein derived from monellin, with potential for food industry applications as a high intensity sweetener. We have been able to sustain this production using a medium based on the cheese whey from the production of ricotta cheese, with minimal pre-treatment of the waste. As a proof of concept, we have also tested these conditions for the production of MMP-9, a protein that had been previously successfully obtained from L. lactis cultures in standard growth conditions.
Conclusions
Other than presenting a new system for the recombinant production of MNEI, more compliant with its potential applications in food industry, our results introduce a strategy to valorize dairy effluents through the synthesis of high added value recombinant proteins. Interestingly, the possibility of using this whey-derived medium relied greatly on the choice of the appropriate codon usage for the target gene. In fact, when a gene optimized for L. lactis was used, the production of MNEI proceeded with good yields. On the other hand, when an E. coli optimized gene was employed, protein synthesis was greatly reduced, to the point of being completely abated in the cheese whey-based medium. The production of MMP-9 was comparable to what observed in the reference conditions.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
663/664 - Food and nutrition. Enology. Oils. Fat
Pages
9
Publisher
BioMed Central
Is part of
Microbial Cell Factories
Citation
Boumaiza, Mohamed, Andrea Colarusso, Ermenegilda Parrilli, Elena Garcia-Fruitós, Angela Casillo, Anna Arís, Maria Michela Corsaro, Delia Picone, Serena Leone, and Maria Luisa Tutino. 2018. "Getting Value From The Waste: Recombinant Production Of A Sweet Protein By Lactococcus Lactis Grown On Cheese Whey". Microbial Cell Factories 17 (1). Springer Nature. doi:10.1186/s12934-018-0974-z.
Grant agreement number
Program
Producció de Remugants
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2694]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/