Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study
Autor/a
Dorantes-Aranda, Juan José
Tan, Jessica Y.C.
Hallegraeff, Gustaaf M.
Campbell, Katrina
Ugalde, Sarah C.
Harwood, D. Tim
Bartlett, Jill K.
Crooks, Steven
Gerssen, Arjen
Harrison, Keith
Huet, Anne-Catherine
Jordan, Timothy B.
Koeberl, Martina
Monaghan, Tim
Murray, Sam
Nimmagadda, Rama
Ooms, Corinne
Quinlan, Rae K.
Shi, Feng
Turner, Andrew D.
Yakes, Betsy Jean
Turnbull, Alison R.
Fecha de publicación
2018-03-01ISSN
1060-3271
Resumen
Paralytic shellfish toxins (PSTs) in bivalve molluscs represent a public health risk and are controlled via compliance with a regulatory limit of 0.8 mg saxitoxin (STX)⋅2HCl equivalents per kilogram of shellfish meat (eq/kg). Shellfish industries would benefit from the use of rapid immunological screening tests for PSTs to be used for regulation, but to date none have been fully validated. An interlaboratory study involving 16 laboratories was performed to determine the suitability of the Neogen test to detect PSTs in mussels and oysters. Participants performed the standard protocol recommended by the manufacturer and a modified protocol with a conversion step to improve detection of gonyautoxin 1&4. The statistical analysis showed that the protocols had good homogeneity across all laboratories, with satisfactory repeatability, laboratory, and reproducibility variation near the regulatory level. The mean probability of detection (POD) at 0.8 mg STX⋅2HCl eq/kg using the standard protocol in mussels and oysters was 0.966 and 0.997, respectively, and 0.968 and 0.966 using the modified protocol. The estimated LOD in mussels was 0.316 mg STX⋅2HCl eq/kg with the standard and 0.682 mg STX⋅2HCl eq/kg with the modified protocol, and 0.710 and 0.734 mg STX⋅2HCl eq/kg for oysters, respectively. The Neogen test may be acceptable for regulatory purposes for oysters in accordance with European Commission directives in which the standard protocol provides, at the regulatory level, a probability of a negative response of 0.033 on 95% of occasions. Its use for mussels is less consistent at the regulatory level due to the wide prediction interval around the POD.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca
Páginas
12
Publicado por
AOAC International
Publicado en
Journal of AOAC International
Citación
Dorantes-Aranda, Juan José, Jessica Y. C. Tan, Gustaaf M. Hallegraeff, Katrina Campbell, Sarah C. Ugalde, D. Tim Harwood, Jill K. Bartlett, Mònica Campàs, Steven Crooks, Arjen Gerssen, Keith Harrison, Anne-Catherine Huet, Timothy B. Jordan, Martina Koeberl, Tim Monaghan, Sam Murray, Rama Nimmagadda, Corinne Ooms, Rae K. Quinlan, Feng Shi, Andrew D. Turner, Betsy Jean Yakes, and Alison R. Turnbull. "Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study." Journal of AOAC International 101, no. 2 (2018): 468-79.
Program
Aigües Marines i Continentals
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