Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools
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Publication date
2020-08-10ISSN
0048-9697
Abstract
Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for the detection of microalgae. In this sense, nucleic acid-based (molecular) methods are being considered for the unequivocal identification of microalgae as an attractive alternative to the currently used time-consuming and laboratory-based light microscopy techniques. This review provides an overview of the progress made on new molecular biotechnological tools for microalgal detection, particularly focusing on those that combine a nucleic acid (DNA or RNA) amplification step with detection. Different types of amplification processes (thermal and isothermal) and detection formats (e.g. microarrays, biosensors, lateral flows) are presented, and a comprehensive overview of their advantages and limitations is provided Although isothermal techniques are an attractive alternative to thermal amplification to reach in-situ analysis, further development is still required. Finally, current challenges, critical steps and future directions of the whole analysis process (from sample procurement to in-situ implementation) are described.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
Pages
27
Publisher
Elsevier
Is part of
Science of The Total Environment
Citation
Toldrà, Anna, Ciara K. O'Sullivan, Jorge Diogène, and Mònica Campàs. 2020. "Detecting Harmful Algal Blooms With Nucleic Acid Amplification-Based Biotechnological Tools". Science Of The Total Environment 749: 141605. doi:10.1016/j.scitotenv.2020.141605.
Grant agreement number
MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanalíticos para detección rápida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSISNG
Program
Aigües Marines i Continentals
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2850]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/