Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads
Ver/Abrir
Autor/a
Toldrà, Anna
Andree, Karl B.
Bertomeu, Edgar
Carrasco, Noèlia
Gairín, Ignasi
Furones, M. Dolores
Fecha de publicación
2018-10-03ISSN
1932-6203
Resumen
Ostreid herpesvirus-1 (OsHV-1) has been involved in mass mortality episodes of Pacific oysters Crassostrea gigas throughout the world, causing important economic losses to the aquaculture industry. In the present study, magnetic beads (MBs) coated with an anionic polymer were used to capture viable OsHV-1 from two types of naturally infected matrix: oyster homogenate and seawater. Adsorption of the virus on the MBs and characterisation of the MB-virus conjugates was demonstrated by real-time quantitative PCR (qPCR). To study the infective capacity of the captured virus, MB-virus conjugates were injected in the adductor muscle of naïve spat oysters, using oyster homogenate and seawater without MBs as positive controls, and bare MBs and sterile water as negative controls. Mortalities were induced after injection with MB-virus conjugates and in positive controls, whereas no mortalities were recorded in negative controls. Subsequent OsHV-1 DNA and RNA analysis of the oysters by qPCR and reverse transcription qPCR (RT-qPCR), respectively, confirmed that the virus was the responsible for the mortality event and the ability of the MBs to capture viable viral particles. The capture of viable OsHV-1 using MBs is a rapid and easy isolation method and a promising tool, combined with qPCR, to be applied to OsHV-1 detection in aquaculture facilities.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
639 - Caza. Pesca. Piscicultura
Páginas
15
Publicado por
Public Library of Science
Publicado en
PLoS ONE
Citación
Toldrà, A., Andree, K., Bertomeu, E., Roque, A., Carrasco, N., & Gairín, I. et al. (2018). Rapid capture and detection of ostreid herpesvirus-1 from Pacific oyster Crassostrea gigas and seawater using magnetic beads. PLOS ONE, 13(10), e0205207. doi:10.1371/journal.pone.0205207
Número del acuerdo de la subvención
EC/H2020/678589/EU/Preventing and mitigating farmed bivalve diseases/VIVALDI
Program
Aigües Marines i Continentals
Aqüicultura
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