A qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studies
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Fecha de publicación
2020-10-17ISSN
0929-1873
Resumen
A qPCR-based method was developed to detect and quantify Podosphaera pannosa, the main causal agent of peach powdery mildew. A primer pair was designed to target part of the ITS region of the fungal ribosomal DNA, which proved to be highly specific and sensitive. A minimum of 2.81 pg µL− 1 of P. pannosa DNA and 6 conidia mL− 1 in artificially-prepared conidia suspensions were found to be the limit of detection. Moreover, a quantification of conidia placed on plastic tapes commonly used in volumetric air samplers was performed. Regression equations on conidia quantification obtained either from aqueous conidia suspensions or conidia placed on plastic tapes were similar. The protocol was further validated in field conditions by estimating the number of P. pannosa conidia obtained with an air sampler, by both microscopic and molecular quantification. Both techniques detected the peaks of conidia production during a 4-month sampling period, and a significant correlation (r = 0.772) was observed between both quantification methods. Additionally, the molecular method was applied to detect latent fungal inoculum in different plant parts of peach trees. The pathogen was detected mainly on the bark of affected twigs, and to a lesser extent, in foliar buds. The method developed here can be applied in the study of P. pannosa epidemiology and can help in improving the management of this pathogen through its early detection and quantification.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
Inglés
Materias (CDU)
632 - Enfermedades y protección de las plantas
Páginas
34
Publicado por
Springer
Publicado en
European Journal of Plant Pathology
Citación
Marimon, Neus, Iban Eduardo, Maela León, Mónica Berbegal, Josep Armengol, and Jordi Luque. 2020. "A Qpcr-Based Method For The Detection And Quantification Of The Peach Powdery Mildew (Podosphaera Pannosa) In Epidemiological Studies". European Journal Of Plant Pathology 158 (4): 1005-1016. doi:10.1007/s10658-020-02136-0.
Número del acuerdo de la subvención
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RTA2013-00004-C03-01/ES/Estrategias de control de enfermedades fúngicas aéreas y de raíz en frutales mediterráneos, basadas en criterios epidemiológicos y en la utilización de micorrizas arbusculares/
Program
Protecció Vegetal Sostenible
Genòmica i Biotecnologia
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