Deletion of the Gene for the Type I Interferon Inhibitor I329L from the Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced in Pigs
Ver/Abrir
Autor/a
Reis, Ana Luisa
Goatley, Lynnette C.
Jabbar, Tamara
Lopez, Elisabeth
Rathakrishnan, Anusyah
Dixon, Linda K.
Fecha de publicación
2020-05-30ISSN
2076-393X
Resumen
Live attenuated vaccines are considered to be the fastest route to the development of a safe and efficacious African swine fever (ASF) vaccine. Infection with the naturally attenuated OURT88/3 strain induces protection against challenge with virulent isolates from the same or closely related genotypes. However, adverse clinical signs following immunisation have been observed. Here, we attempted to increase the OURT88/3 safety profile by deleting I329L, a gene previously shown to inhibit the host innate immune response. The resulting virus, OURT88/3ΔI329L, was tested in vitro to evaluate the replication and expression of type I interferon (IFN) and in vivo by immunisation and lethal challenge experiments in pigs. No differences were observed regarding replication; however, increased amounts of both IFN-β and IFN-α were observed in macrophages infected with the deletion mutant virus. Unexpectedly, the deletion of I329L markedly reduced protection against challenge with the virulent OURT88/1 isolate. This was associated with a decrease in both antibody levels against VP72 and the number of IFN-γ-producing cells in the blood of non-protected animals. Furthermore, a significant increase in IL-10 levels in serum was observed in pigs immunised with OURT88/3ΔI329L following challenge. Interestingly, the deletion of the I329L gene failed to attenuate the virulent Georgia/2007 isolate.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
15
Publicado por
MDPI
Publicado en
Vaccines
Citación
Reis, Ana Luisa, Lynnette C. Goatley, Tamara Jabbar, Elisabeth Lopez, Anusyah Rathakrishnan, and Linda K. Dixon. 2020. "Deletion Of The Gene For The Type I Interferon Inhibitor I329L From The Attenuated African Swine Fever Virus OURT88/3 Strain Reduces Protection Induced In Pigs". Vaccines 8 (2): 262. doi:10.3390/vaccines8020262.
Número del acuerdo de la subvención
EC/FP7/311931/EU/Targeted research effort on African swine fever/ASFORCE
Program
Sanitat Animal
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