Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR
Visualitza/Obre
Autor/a
Macedo, Nubia
Gottschalk, Marcelo
Strutzberg-Minder, Katrin
Van, Chao Nguyen
Zhang, Lijun
Zou, Geng
Zhou, Rui
Marostica, Thaire
Clavijo, Maria Jose
Tucker, Alexander
Data de publicació
2021-05-12ISSN
0928-4249
Resum
Glaesserella parasuis strains were characterized by serotyping PCR, vtaA virulence marker Leader Sequence (LS)-PCR, clinical significance, and geographic region. Overall, the serovars 4, 5/12, 7, 1, and 13 were the most commonly detected. Serovars of greatest clinical relevance were systemic isolates that had a higher probability of being serovar 5/12, 13, or 7. In comparison, pulmonary isolates had a higher likelihood of being serovars 2, 4, 7, or 14. Serovars 5/12 and 13 have previously been considered disease-associated, but this study agrees with other recent studies showing that serovar 7 is indeed associated with systemic G. parasuis disease. Serovar 4 strains illustrated how isolates can have varying degrees of virulence and be obtained from pulmonary, systemic, or nasal sites. Serovars 8, 9, 15, and 10 were predominantly obtained from nasal samples, which indicates a limited clinical significance of these serovars. Additionally, most internal G. parasuis isolates were classified as virulent by LS-PCR and were disease-associated isolates, including serovars 1, 2, 4, 5/12, 7, 13, and 14. Isolates from the nasal cavity, including serovars 6, 9, 10, 11, and 15, were classified as non-virulent by LS-PCR. In conclusion, the distribution of G. parasuis serovars remains constant, with few serovars representing most of the strains isolated from affected pigs. Moreover, it was confirmed that the LS-PCR can be used for G. parasuis virulence prediction of field strains worldwide.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
10
Publicat per
BMC
Publicat a
Veterinary Research
Citació
Macedo, Nubia, Marcelo Gottschalk, Katrin Strutzberg-Minder, Chao Nguyen Van, Lijun Zhang, Geng Zou, and Rui Zhou et al. 2021. "Molecular Characterization Of Glaesserella Parasuis Strains Isolated From North America, Europe And Asia By Serotyping PCR And LS-PCR". Veterinary Research 52 (1). doi:10.1186/s13567-021-00935-9.
Programa
Sanitat Animal
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