Identification and Characterization of Swine Influenza Virus H1N1 Variants Generated in Vaccinated and Nonvaccinated, Challenged Pigs
Autor/a
López-Valiñas, Álvaro
Sisteré-Oró, Marta
Baioni, Laura
Chiapponi, Chiara
Núñez, José I.
Fecha de publicación
2021-10-16ISSN
1999-4915
Resumen
Influenza viruses represent a continuous threat to both animal and human health. The 2009 H1N1 A influenza pandemic highlighted the importance of a swine host in the adaptation of influenza viruses to humans. Nowadays, one of the most extended strategies used to control swine influenza viruses (SIVs) is the trivalent vaccine application, whose formulation contains the most frequently circulating SIV subtypes H1N1, H1N2, and H3N2. These vaccines do not provide full protection against the virus, allowing its replication, evolution, and adaptation. To better understand the main mechanisms that shape viral evolution, here, the SIV intra-host diversity was analyzed in samples collected from both vaccinated and nonvaccinated animals challenged with the H1N1 influenza A virus. Twenty-eight whole SIV genomes were obtained by next-generation sequencing, and differences in nucleotide variants between groups were established. Substitutions were allocated along all influenza genetic segments, while the most relevant nonsynonymous substitutions were allocated in the NS1 protein on samples collected from vaccinated animals, suggesting that SIV is continuously evolving despite vaccine application. Moreover, new viral variants were found in both vaccinated and nonvaccinated pigs, showing relevant substitutions in the HA, NA, and NP proteins, which may increase viral fitness under field conditions.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
18
Publicado por
MDPI
Publicado en
Viruses
Citación
López-Valiñas, Álvaro , Marta Sisteré-Oró, Sergi López-Serrano, Laura Baioni, Ayub Darji, Chiara Chiapponi, Joaquim Segalés, Llilianne Ganges and José I. Núñez. Identification and Characterization of Swine Influenza Virus H1N1 Variants Generated in Vaccinated and Nonvaccinated, Challenged Pigs. Viruses 2021, 13, 2087. https://doi.org/10.3390/v13102087
Número del acuerdo de la subvención
MINECO/Programa Estatal de I+D+I orientada a los retos de la Sociedad/AGL2016-75280-R/ES/EVOLUCION DEL VIRUS INFLUENZA PORCINA ASOCIADA A LA VACUNACION/
Program
Sanitat Animal
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