Comparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strains
Visualitza/Obre
Autor/a
Herrera-Uribe, Júber
Jiménez-Marín, Ángeles
Lacasta, Anna
L. Monteagudo, Paula
Rodríguez, Fernando
Moreno, Ángela
Garrido, Juan J.
Data de publicació
2018-09-12ISSN
0928-4249
Resum
African swine fever (ASF) is a pathology of pigs against which there is no treatment or vaccine. Understanding the
equilibrium between innate and adaptive protective responses and immune pathology might contribute to the
development of strategies against ASFV. Here we compare, using a proteomic approach, the course of the in vivo
infection caused by two homologous strains: the virulent E75 and the attenuated E75CV1. Our results show a progres‑
sive loss of proteins by day 7 post-infection (pi) with E75, refecting tissue destruction. Many signal pathways were
afected by both infections but in diferent ways and extensions. Cytoskeletal remodelling and clathrin-endocytosis
were afected by both isolates, while a greater number of proteins involved on infammatory and immunological
pathways were altered by E75CV1. 14-3-3 mediated signalling, related to immunity and apoptosis, was inhibited
by both isolates. The implication of the Rho GTPases by E75CV1 throughout infection is also evident. Early events
refected the lack of E75 recognition by the immune system, an evasion strategy acquired by the virulent strains, and
signifcant changes at 7 days post-infection (dpi), coinciding with the peak of infection and the time of death. The
protein signature at day 31 pi with E75CV1 seems to refect events observed at 1 dpi, including the upregulation of
proteosomal subunits and molecules described as autoantigens (vimentin, HSPB1, enolase and lymphocyte cytosolic
protein 1), which allow the speculation that auto-antibodies could contribute to chronic ASFV infections. Therefore,
the use of proteomics could help understand ASFV pathogenesis and immune protection, opening new avenues for
future research.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
15
Publicat per
BioMed Central
Publicat a
Veterinary Research
Citació
Herrera-Uribe, Júber, Ángeles Jiménez-Marín, Anna Lacasta, Paula L. Monteagudo, Sonia Pina-Pedrero, Fernando Rodríguez, Ángela Moreno, and Juan J. Garrido. 2018. "Comparative Proteomic Analysis Reveals Different Responses In Porcine Lymph Nodes To Virulent And Attenuated Homologous African Swine Fever Virus Strains". Veterinary Research 49 (1). Springer Nature America, Inc. doi:10.1186/s13567-018-0585-z.
Número de l'acord de la subvenció
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229-C03-01/ES/Caracterización de los mecanismos inmunológicos implicados en protección frente al virus de la peste porcina africana: desarrollo de vacuna frente al VPPA/
MICINN/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-87415-R/ES/Análisis de la interacción patógeno-microbiota-hospedador para entender los mecanismos de colonización y persistencia de Salmonella en el intestino porcino/
Programa
Sanitat Animal
Aquest element apareix en la col·lecció o col·leccions següent(s)
- ARTICLES CIENTÍFICS [2549]
Els següents fitxers sobre la llicència estan associats a aquest element:
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com http://creativecommons.org/licenses/by/4.0/