Gut‑associated microbes are present and active in the pig nasal cavity
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Author
Publication date
2024-04-11ISSN
2045-2322
Abstract
The nasal microbiota is a key contributor to animal health, and characterizing the nasal microbiota
composition is an important step towards elucidating the role of its diferent members. Eforts to
characterize the nasal microbiota composition of domestic pigs and other farm animals frequently
report the presence of bacteria that are typically found in the gut, including many anaerobes from the
Bacteroidales and Clostridiales orders. However, the in vivo role of these gut‑microbiota associated
taxa is currently unclear. Here, we tackled this issue by examining the prevalence, origin, and activity
of these taxa in the nasal microbiota of piglets. First, analysis of the nasal microbiota of farm piglets
sampled in this study, as well as various publicly available data sets, revealed that gut‑microbiota
associated taxa indeed constitute a substantial fraction of the pig nasal microbiota that is highly
variable across individual animals. Second, comparison of herd‑matched nasal and rectal samples at
amplicon sequencing variant (ASV) level showed that these taxa are largely shared in the nasal and
rectal microbiota, suggesting a common origin driven presumably by the transfer of fecal matter.
Third, surgical sampling of the inner nasal tract showed that gut‑microbiota associated taxa are found
throughout the nasal cavity, indicating that these taxa do not stem from contaminations introduced
during sampling with conventional nasal swabs. Finally, analysis of cDNA from the 16S rRNA gene in
these nasal samples indicated that gut‑microbiota associated taxa are indeed active in the pig nasal
cavity. This study shows that gut‑microbiota associated taxa are not only present, but also active,
in the nasal cavity of domestic pigs, and paves the way for future eforts to elucidate the function of
these taxa within the nasal microbiota.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
11
Publisher
Nature Research
Is part of
Scientific Reports
Citation
Obregon-Gutierrez, Pau, Laura Bonillo-Lopez, Florencia Correa‐Fiz, Marina Sibila, Joaquím Segalés, Karl Kochanowski, and Virginia Aragón. 2024. “Gut-associated Microbes Are Present and Active in the Pig Nasal Cavity.” Scientific Reports 14 (1): 8470. doi:10.1038/s41598-024-58681-9
Grant agreement number
MICINN/Programa Estatal para desarrollar, atraer y retener talento/RYC2021-033035-I/ES/ /
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-106233RB-I00/ES/FUNCIONALIDAD Y EFECTO DE LA MICROBIOTA NASAL SOBRE LA RESPUESTA INMUNE DE LOS LECHONES/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2838]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/