Development and validation of direct PCR and quantitative PCR assays for the rapid, sensitive, and economical detection of porcine circovirus 3
Ver/Abrir
Autor/a
Franzo, Giovanni
Legnardi, Matteo
Centelleghe, Cinzia
Tucciarone, Claudia M.
Cecchinato, Mattia
Cortey, Martí
Drigo, Michele
Fecha de publicación
2018-04-09ISSN
1040-6387
Resumen
Since the identification of species Porcine circovirus 2, the relevance of genus Circovirus has increased given its impact on the swine industry. A new species (Porcine circovirus 3, PCV-3) has been detected in association with various clinical conditions. Consequently, there is an urgent need for reliable and widely accessible tests for both routine diagnostic and research purposes. We developed a direct PCR (requiring no DNA extraction) and a quantitative (q)PCR targeting the conserved rep gene to detect the PCV-3 genome. Test performance was assessed by testing 120 field samples within different matrices. Both methods were sensitive (detection of 10 viral genome/µL), specific, and repeatable. The substantially perfect agreement between the 2 assays strongly supports their high sensitivity and specificity. The low cost and short processing time of the direct PCR protocol, together with the reliable quantitative results provided by qPCR, support the establishment of common testing guidelines.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
Inglés
Materias (CDU)
619 - Veterinaria
Páginas
25
Publicado por
SAGE Publications
Publicado en
Journal of Veterinary Diagnostic Investigation
Citación
Franzo, Giovanni, Matteo Legnardi, Cinzia Centelleghe, Claudia M. Tucciarone, Mattia Cecchinato, Martí Cortey, Joaquim Segalés, and Michele Drigo. “Development and Validation of Direct PCR and Quantitative PCR Assays for the Rapid, Sensitive, and Economical Detection of Porcine Circovirus 3.” Journal of Veterinary Diagnostic Investigation 30, no. 4 (July 2018): 538–44. doi:10.1177/1040638718770495.
Program
Sanitat Animal
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