Analysing the Expression of Eight Clock Genes in Five Tissues From Fasting and Fed Sows
Visualitza/Obre
Autor/a
Cardoso, Tainã Figueiredo
Castelló, Anna
Mármol-Sánchez, Emilio
Ballester, Maria
Jordana, Jordi
Amills, Marcel
Data de publicació
2018-10-18ISSN
1664-8021
Resum
In a previous study, we observed that circadian clock genes are differentially expressed
in the skeletal muscle of fasting and fed sows. The goal of the current work was
to investigate if these genes are also differentially expressed in tissues containing the
central (hypothalamus) and peripheral (duodenum, dorsal fat, muscle, and liver) clocks.
As animal material, we used 12 sows that fasted 12 h before slaughtering (T0) and
12 sows that were fed ad libitum 7 h prior slaughtering (T2). Tissue samples were
collected immediately after slaughter and total RNA was subsequently extracted. The
expression of the ARNTL, BHLHE40, CRY2, NPAS2, NR1D1, PER1, PER2, and SIK1
genes was measured by quantitative reverse transcription PCR. The numbers of clock
genes showing differential expression before and after feeding varied depending on the
tissue i.e., four in dorsal fat and duodenum, six in skeletal muscle, and seven in the
liver. In contrast, none of the eight analysed genes displayed a significant differential
expression in hypothalamus, the tissue where the central clock resides. This result
supports that the differential expression of clock genes in the four tissues mentioned
above is probably induced by nutrition and not by the central clock entrained by light.
Moreover, we have observed that the NPAS2 and ARNTL genes display positive log2(FC)
values in the five tissues under analysis, whilst the CRY2, PER1 (except dorsal fat)
and PER2 (except hypothalamus) genes generally show negative log2(FC) values. Such
result might be explained by the existence of a negative feedback loop between the
ARNTL/NPAS2 and CRY/PER genes. Collectively, these results support that nutrition
plays an important role in modulating the timing of porcine peripheral circadian clocks.
Such regulation could be essential for coordinating the subsequent metabolic response
to nutrient supply.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
8
Publicat per
Frontiers Media
Publicat a
Frontiers in Genetics
Citació
Cardoso, Tainã Figueiredo, Raquel Quintanilla, Anna Castelló, Emilio Mármol-Sánchez, Maria Ballester, Jordi Jordana, and Marcel Amills. 2018. "Analysing The Expression Of Eight Clock Genes In Five Tissues From Fasting And Fed Sows". Frontiers In Genetics 9. Frontiers Media SA. doi:10.3389/fgene.2018.00475.
Número de l'acord de la subvenció
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48742-C2-1-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48742-C2-2-R/ES/FISIOLOGIA GENOMICA DEL DEPOSITO DE GRASA INTRAMUSCULAR EN PORCINO/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ /
Programa
Genètica i Millora Animal
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