Now showing items 1-4 of 4
Avoiding quantification bias in metabarcoding: Application of a cell biovolume correction factor in diatom molecular biomonitoring
(Methods in Ecology and Evolution, 2017-12-26)
In recent years, remarkable progress has been made in developing environmental DNA metabarcoding. However, its ability to quantify species relative abundance remains uncertain, limiting its application ...
Diat.barcode, an open-access curated barcode library for diatoms
(Scientific Reports, 2019-10-22)
Diatoms (Bacillariophyta) are ubiquitous microalgae which produce a siliceous exoskeleton and which make a major contribution to the productivity of oceans and freshwaters. They display a huge diversity, ...
Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life
(Metabarcoding and Metagenomics (MBMG), 2021-02-26)
DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode ...
The potential of High-Throughput Sequencing (HTS) of natural samples as a source of primary taxonomic information for reference libraries of diatom barcodes
Diatoms are used routinely to assess pollution level in rivers and lakes. Current methods are based on identification by light microscopy, which is laborious. An alternative is to identify species based ...