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Impact of Cryopreservation on Viability, Phenotype, and Functionality of Porcine PBMC
(Frontiers Media, 2021-11-29)
The use of frozen peripheral blood mononuclear cells (PBMC) is common in immunological studies. The impact of freezing PBMC has been assessed using human and mice cells, but little information is available ...
A retrospective study of porcine epidemic diarrhoea virus (PEDV) reveals the presence of swine enteric coronavirus (SeCoV) since 1993 and the recent introduction of a recombinant PEDV‐SeCoV in Spain
(Wiley, 2020-06-08)
A retrospective evaluation of PEDV positive samples recovered in Spain before and after the re‐emergence of this coronavirus in several European countries was carried out. We described for the first ...
Rules of thumb to obtain, isolate, and preserve porcine peripheral blood mononuclear cells
(Elsevier, 2022-07-16)
One of the most used biospecimens in immunology are peripheral blood mononuclear cells (PBMC). PBMC are particularly useful when evaluating immunity through responses of circulating B- and T-cells, ...
Assessment of three commercial ELISAs for the detection of antibodies against Porcine epidemic diarrhea virus at different stages of the immune response
(Elsevier, 2021-02-12)
Three commercial ELISAs -two based on spike (E1 and E3) and one on nucleocapsid protein (E2)-were used to analyze the development and persistence of antibodies against Porcine epidemic diarrhea virus ...